HAS1 / HAS Antibody
Catalog Number: LS-C497162
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human HAS1 / HAS Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G80997-20
Catalog Number: LS-G80997
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Hyaluronan synthase 1(HAS1) (NM_001523) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HAS1 | HAS | Hyaluronan synthase 1 | Hyaluronic acid synthase 1 | HuHAS1 | HA synthase 1 | Hyaluronate synthase 1
- Origin Species
- Human
- NCBI GenBank
- NM_001523
- SwissProt
- Q92839
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRQDAPKPTP AARRCSGLAR RVLTIAFALL ILGLMTWAYA AGVPLASDRY GLLAFGLYGA61FLSAHLVAQS LFAYLEHRRV AAAARGPLDA ATARSVALTI SAYQEDPAYL RQCLASARAL121LYPRARLRVL MVVDGNRAED LYMVDMFREV FADEDPATYV WDGNYHQPWE PAAAGAVGAG181AYREVEAEDP GRLAVEALVR TRRCVCVAQR WGGKREVMYT AFKALGDSMD YVQVCDSDTR241LDPMALLELV RVLDEDPRVG AVGGDVRILN PLDSWVSFLS SLRYWVAFNV ERACQSYFHC301VSCISGPLGL YRNNLLQQFL EAWYNQKFLG THCTFGDDRH LTNRMLSMGY ATKYTSRSRC361YSETPSSFLR WLSQQTRWSK SYFREWLYNA LWWHRHHAWM TYEAVVSGLF PFFVAATVLR421LFYAGRPWAL LWVLLCVQGV ALAKAAFAAW LRGCLRMVLL SLYAPLYMCG LLPAKFLALV481TMNQSGWGTS GRRKLAANYV PLLPLALWAL LLLGGLVRSV AHEARADWSG PSRAAEAYHL541AAGAGAYVGY WVAMLTLYWV GVRRLCRRRT GGYRVQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
577 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
