Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HAS1 / HAS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118534-100
Catalog Number: LS-G118534
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hyaluronan synthase 1(HAS1) (NM_001523), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HAS1 | HAS | Hyaluronan synthase 1 | Hyaluronic acid synthase 1 | HuHAS1 | HA synthase 1 | Hyaluronate synthase 1
- Origin Species
- Human
- NCBI GenBank
- NM_001523
- SwissProt
- Q92839
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRQDAPKPTP AARRCSGLAR RVLTIAFALL ILGLMTWAYA AGVPLASDRY GLLAFGLYGA61FLSAHLVAQS LFAYLEHRRV AAAARGPLDA ATARSVALTI SAYQEDPAYL RQCLASARAL121LYPRARLRVL MVVDGNRAED LYMVDMFREV FADEDPATYV WDGNYHQPWE PAAAGAVGAG181AYREVEAEDP GRLAVEALVR TRRCVCVAQR WGGKREVMYT AFKALGDSMD YVQVCDSDTR241LDPMALLELV RVLDEDPRVG AVGGDVRILN PLDSWVSFLS SLRYWVAFNV ERACQSYFHC301VSCISGPLGL YRNNLLQQFL EAWYNQKFLG THCTFGDDRH LTNRMLSMGY ATKYTSRSRC361YSETPSSFLR WLSQQTRWSK SYFREWLYNA LWWHRHHAWM TYEAVVSGLF PFFVAATVLR421LFYAGRPWAL LWVLLCVQGV ALAKAAFAAW LRGCLRMVLL SLYAPLYMCG LLPAKFLALV481TMNQSGWGTS GRRKLAANYV PLLPLALWAL LLLGGLVRSV AHEARADWSG PSRAAEAYHL541AAGAGAYVGY WVAMLTLYWV GVRRLCRRRT GGYRVQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
577 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.