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Human GSE1 / KIAA0182 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GSE1 / KIAA0182 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G76688-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GSE1 (NM_014615) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Gse1 coiled-coil protein | GSE1 | KIAA0182
Origin Species
Human
NCBI GenBank
D80004
SwissProt
Q14687

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGMSHEPKS PSLGMLSTAT RTTATVNPLT PSPLNGALVP SGSPATSSAL SAQAAPSSSF
61AAALRKLAKQ AEEPRGSSLS SESSPVSSPA TNHSSPASTP KRVPMGPIIV PPGGHSVPST
121PPVVTIAPTK TVNGVWRSES RQDAGSRSSS GGRERLIVEP PLPQEKAGGP AIPSHLLSTP
181YPFGLSPSSV VQDSRFPPLN LQRPVHHVVP PSTVTEDYLR SFRPYHTTDD LRMSSLPPLG
241LDPATAAAYY HPSYLAPHPF PHPAFRMDDS YCLSALRSPF YPIPTPGSLP PLHPSAMHLH
301LSGVRYPPEL SHSSLAALHS ERMSGLSAER LQMDEELRRE RERERERERE READREREKE
361REREREKERE QEKEREREKE RERELERQRE QRAREKELLA AKALEPSFLP VAELHGLRGH
421ATEERGKPSE QLTPTRAEKL KDAGLQAPKP VQHPLHPVPT PHHTVPSLIS NHGIFSLPSS
481SAATALLIQR TNEEEKWLAR QRRLRQEKED RQSQVSEFRQ QVLEQHLDMG RPPVPAEAEH
541RPESTTRPGP NRHEPGGRDP PQHFGGPPPL ISPKPQLHAA PTALWNPVSL MDNTLETRRA
601ESHSLHSHPA AFEPSRQAAV PLVKVERVFC PEKAEEGPRK REPAPLDKYQ PPPPPPREGG
661SLEHQPFLPG PGPFLAELEK STQTILGQQR ASLPQAATFG ELSGPLKPGS PYRPPVPRAP
721DPAYIYDEFL QQRRRLVSKL DLEERRRREA QEKGYYYDLD DSYDESDEEE VRAHLRCVAE
781QPPLKLDTSS EKLEFLQLFG LTTQQQKEEL VAQKRRKRRR MLRERSPSPP TIQSKRQTPS
841PRLALSTRYS PDEMNNSPNF EEKKKFLTIF NLTHISAEKR KDKERLVEML RAMKQKALSA
901AVADSLTNSP RDSPAVSLSE PATQQASLDV EKPVGVAASL SDIPKAAEPG KLEQVRPQEL
961SRVQELAPAS GEKARLSEAP GGKKSLSMLH YIRGAAPKDI PVPLSHSTNG KSKPWEPFVA
1021EEFAHQFHES VLQSTQKALQ KHKGSVAVLS AEQNHKVDTS VHYNIPELQS SSRAPPPQHN
1081GQQEPPTARK GPPTQELDRD SEEEEEEDDE DGEDEEEVPK RKWQGIEAVF EAYQEHIEEQ
1141NLERQVLQTQ CRRLEARHYS LSLTAEQLSH SVAELRSQKQ KMVSERERLQ AELDHLRKCL
1201ALPAMHWPRG YLKGYPR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1217 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.