Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GSE1 / KIAA0182 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113833-100
Catalog Number: LS-G113833
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GSE1 (NM_014615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gse1 coiled-coil protein | GSE1 | KIAA0182
- Origin Species
- Human
- NCBI GenBank
- D80004
- SwissProt
- Q14687
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 136 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKGMSHEPKS PSLGMLSTAT RTTATVNPLT PSPLNGALVP SGSPATSSAL SAQAAPSSSF61AAALRKLAKQ AEEPRGSSLS SESSPVSSPA TNHSSPASTP KRVPMGPIIV PPGGHSVPST121PPVVTIAPTK TVNGVWRSES RQDAGSRSSS GGRERLIVEP PLPQEKAGGP AIPSHLLSTP181YPFGLSPSSV VQDSRFPPLN LQRPVHHVVP PSTVTEDYLR SFRPYHTTDD LRMSSLPPLG241LDPATAAAYY HPSYLAPHPF PHPAFRMDDS YCLSALRSPF YPIPTPGSLP PLHPSAMHLH301LSGVRYPPEL SHSSLAALHS ERMSGLSAER LQMDEELRRE RERERERERE READREREKE361REREREKERE QEKEREREKE RERELERQRE QRAREKELLA AKALEPSFLP VAELHGLRGH421ATEERGKPSE QLTPTRAEKL KDAGLQAPKP VQHPLHPVPT PHHTVPSLIS NHGIFSLPSS481SAATALLIQR TNEEEKWLAR QRRLRQEKED RQSQVSEFRQ QVLEQHLDMG RPPVPAEAEH541RPESTTRPGP NRHEPGGRDP PQHFGGPPPL ISPKPQLHAA PTALWNPVSL MDNTLETRRA601ESHSLHSHPA AFEPSRQAAV PLVKVERVFC PEKAEEGPRK REPAPLDKYQ PPPPPPREGG661SLEHQPFLPG PGPFLAELEK STQTILGQQR ASLPQAATFG ELSGPLKPGS PYRPPVPRAP721DPAYIYDEFL QQRRRLVSKL DLEERRRREA QEKGYYYDLD DSYDESDEEE VRAHLRCVAE781QPPLKLDTSS EKLEFLQLFG LTTQQQKEEL VAQKRRKRRR MLRERSPSPP TIQSKRQTPS841PRLALSTRYS PDEMNNSPNF EEKKKFLTIF NLTHISAEKR KDKERLVEML RAMKQKALSA901AVADSLTNSP RDSPAVSLSE PATQQASLDV EKPVGVAASL SDIPKAAEPG KLEQVRPQEL961SRVQELAPAS GEKARLSEAP GGKKSLSMLH YIRGAAPKDI PVPLSHSTNG KSKPWEPFVA1021EEFAHQFHES VLQSTQKALQ KHKGSVAVLS AEQNHKVDTS VHYNIPELQS SSRAPPPQHN1081GQQEPPTARK GPPTQELDRD SEEEEEEDDE DGEDEEEVPK RKWQGIEAVF EAYQEHIEEQ1141NLERQVLQTQ CRRLEARHYS LSLTAEQLSH SVAELRSQKQ KMVSERERLQ AELDHLRKCL1201ALPAMHWPRG YLKGYPRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1217 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.