LSBio
Human GPSM2 / LGN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GPSM2 / LGN Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GPSM2 antibodies →
$153.00each
Catalog Number: LS-G81649-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPSM2 (NM_013296) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DFNB82 | LGN | PINS | CMCS | GPSM2 | Mosaic protein LGN
Origin Species
Human
NCBI GenBank
NM_013296
SwissProt
P81274

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MREDHSFHVR YRMEASCLEL ALEGERLCKS GDCRAGVSFF EAAVQVGTED LKTLSAIYSQ
61LGNAYFYLHD YAKALEYHHH DLTLARTIGD QLGEAKASGN LGNTLKVLGN FDEAIVCCQR
121HLDISRELND KVGEARALYN LGNVYHAKGK SFGCPGPQDV GEFPEEVRDA LQAAVDFYEE
181NLSLVTALGD RAAQGRAFGN LGNTHYLLGN FRDAVIAHEQ RLLIAKEFGD KAAERRAYSN
241LGNAYIFLGE FETASEYYKK TLLLARQLKD RAVEAQSCYS LGNTYTLLQD YEKAIDYHLK
301HLAIAQELND RIGEGRACWS LGNAYTALGN HDQAMHFAEK HLEISREVGD KSGELTARLN
361LSDLQMVLGL SYSTNNSIMS ENTEIDSSLN GVRPKLGRRH SMENMELMKL TPEKVQNWNS
421EILAKQKPLI AKPSAKLLFV NRLKGKKYKT NSSTKVLQDA SNSIDHRIPN SQRKISADTI
481GDEGFFDLLS RFQSNRMDDQ RCCLQEKNCH TASTTTSSTP PKMMLKTSSV PVVSPNTDEF
541LDLLASSQSR RLDDQRASFS NLPGLRLTQN SQSVLSHLMT NDNKEADEDF FDILVKCQGS
601RLDDQRCAPP PATTKGPTVP DEDFFSLILR SQGKRMDEQR VLLQRDQNRD TDFGLKDFLQ
661NNALLEFKNS GKKSADH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

677 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.