Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPSM2 / LGN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114345-100
Catalog Number: LS-G114345
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPSM2 (NM_013296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DFNB82 | LGN | PINS | CMCS | GPSM2 | Mosaic protein LGN
- Origin Species
- Human
- NCBI GenBank
- NM_013296
- SwissProt
- P81274
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MREDHSFHVR YRMEASCLEL ALEGERLCKS GDCRAGVSFF EAAVQVGTED LKTLSAIYSQ61LGNAYFYLHD YAKALEYHHH DLTLARTIGD QLGEAKASGN LGNTLKVLGN FDEAIVCCQR121HLDISRELND KVGEARALYN LGNVYHAKGK SFGCPGPQDV GEFPEEVRDA LQAAVDFYEE181NLSLVTALGD RAAQGRAFGN LGNTHYLLGN FRDAVIAHEQ RLLIAKEFGD KAAERRAYSN241LGNAYIFLGE FETASEYYKK TLLLARQLKD RAVEAQSCYS LGNTYTLLQD YEKAIDYHLK301HLAIAQELND RIGEGRACWS LGNAYTALGN HDQAMHFAEK HLEISREVGD KSGELTARLN361LSDLQMVLGL SYSTNNSIMS ENTEIDSSLN GVRPKLGRRH SMENMELMKL TPEKVQNWNS421EILAKQKPLI AKPSAKLLFV NRLKGKKYKT NSSTKVLQDA SNSIDHRIPN SQRKISADTI481GDEGFFDLLS RFQSNRMDDQ RCCLQEKNCH TASTTTSSTP PKMMLKTSSV PVVSPNTDEF541LDLLASSQSR RLDDQRASFS NLPGLRLTQN SQSVLSHLMT NDNKEADEDF FDILVKCQGS601RLDDQRCAPP PATTKGPTVP DEDFFSLILR SQGKRMDEQR VLLQRDQNRD TDFGLKDFLQ661NNALLEFKNS GKKSADHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
677 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.