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Human GPS1 / CSN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GPS1 / CSN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GPS1 antibodies →
$153.00each
Catalog Number: LS-G75997-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CSN1(GPS1) (NM_212492) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COPS1 | CSN1 | G protein pathway suppressor 1 | GPS-1 | GPS1 | SGN1 | Signalosome subunit 1 | Protein MFH
Origin Species
Human
NCBI GenBank
NM_004127
SwissProt
Q13098

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MRDSSAPSSA SSSVTDLYCT PHSSRSDLVL PGTAGDFSLS ASLSACTLLY EGAVEPMQID
61VDPQEDPQNA PDVNYVVENP SLDLEQYAAS YSGLMRIERL QFIADHCPTL RVEALKMALS
121FVQRTFNVDM YEEIHRKLSE ATRELQNAPD AIPESGVEPP ALDTAWVEAT RKKALLKLEK
181LDTDLKNYKG NSIKESIRRG HDDLGDHYLD CGDLSNALKC YSRARDYCTS AKHVINMCLN
241VIKVSVYLQN WSHVLSYVSK AESTPEIAEQ RGERDSQTQA ILTKLKCAAG LAELAARKYK
301QAAKCLLLAS FDHCDFPELL SPSNVAIYGG LCALATFDRQ ELQRNVISSS SFKLFLELEP
361QVRDIIFKFY ESKYASCLKM LDEMKDNLLL DMYLAPHVRT LYTQIRNRAL IQYFSPYVSA
421DMHRMAAAFN TTVAALEDEL TQLILEGLIS ARVDSHSKIL YARDVDQRST TFEKSLLMGK
481EFQRRAKAMM LRAAVLRNQI HVKSPPREGS QGELTPANSQ SRMSTNM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

527 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.