Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPS1 / CSN1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102745-100
Catalog Number: LS-G102745
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSN1(GPS1) (NM_212492), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COPS1 | CSN1 | G protein pathway suppressor 1 | GPS-1 | GPS1 | SGN1 | Signalosome subunit 1 | Protein MFH
- Origin Species
- Human
- NCBI GenBank
- NM_004127
- SwissProt
- Q13098
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRDSSAPSSA SSSVTDLYCT PHSSRSDLVL PGTAGDFSLS ASLSACTLLY EGAVEPMQID61VDPQEDPQNA PDVNYVVENP SLDLEQYAAS YSGLMRIERL QFIADHCPTL RVEALKMALS121FVQRTFNVDM YEEIHRKLSE ATRELQNAPD AIPESGVEPP ALDTAWVEAT RKKALLKLEK181LDTDLKNYKG NSIKESIRRG HDDLGDHYLD CGDLSNALKC YSRARDYCTS AKHVINMCLN241VIKVSVYLQN WSHVLSYVSK AESTPEIAEQ RGERDSQTQA ILTKLKCAAG LAELAARKYK301QAAKCLLLAS FDHCDFPELL SPSNVAIYGG LCALATFDRQ ELQRNVISSS SFKLFLELEP361QVRDIIFKFY ESKYASCLKM LDEMKDNLLL DMYLAPHVRT LYTQIRNRAL IQYFSPYVSA421DMHRMAAAFN TTVAALEDEL TQLILEGLIS ARVDSHSKIL YARDVDQRST TFEKSLLMGK481EFQRRAKAMM LRAAVLRNQI HVKSPPREGS QGELTPANSQ SRMSTNMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.