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Human GPR50 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GPR50 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GPR50 antibodies →
$153.00each
Catalog Number: LS-G77304-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Melatonin Related Receptor(GPR50) (NM_004224) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 50 | Mel1c | GPR50 | Melatonin-related receptor | MTNRL | H9
Origin Species
Human
NCBI GenBank
NM_004224
SwissProt
Q13585

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPTLAVPTP YGCIGCKLPQ PEYPPALIIF MFCAMVITIV VDLIGNSMVI LAVTKNKKLR
61NSGNIFVVSL SVADMLVAIY PYPLMLHAMS IGGWDLSQLQ CQMVGFITGL SVVGSIFNIV
121AIAINRYCYI CHSLQYERIF SVRNTCIYLV ITWIMTVLAV LPNMYIGTIE YDPRTYTCIF
181NYLNNPVFTV TIVCIRFVLP LLIVGFCYVR IWTKVLAARD PAGQNPDNQL AEVRNFLTMF
241VIFLLFAVCW CPINVLTVLV AVSPKEMAGK TPNWLYLAAY FIAYFNSCLN AVIYGLLNEN
301FRREYWTIFH AMRHPIIFFS GLISDIREMQ EARTLARARA YARDQAREQD RAHACPAVEE
361TPMNVRNVPL PGDAAAGHPD RASGHPKPHS RSSSAYRKSA STHHKSVFSH SKAASGHLKP
421VSGHSKPASG HPKSATVYPK PASVHFKADS VHFKGDSVHF KPDSVHFKPA SSNPKPITGH
481HVSAGSHSKS AFSAATSHPK PTTGHIKPAT SHAEPTTADY PKPATTSHPK PAAADNPELS
541ASHCPEIPAI AHPVSDDSDL PESASSPAAG PTKPAASQLE SDTIADLPDP TVVTTSTNDY
601HDVVVVDVED DPDEMAV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

617 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.