GPR158 Antibody
Catalog Number: LS-C675174
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GPR158 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G92816-20
Catalog Number: LS-G92816
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR158 (NM_020752) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1136 | G protein-coupled receptor 158 | GPR158 | RP11-59G22.1
- Origin Species
- Human
- NCBI GenBank
- NM_020752
- SwissProt
- Q5T848
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 135.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAMAYPLLL CLLLAQLGLG AVGASRDPQG RPDSPRERTP KGKPHAQQPG RASASDSSAP61WSRSTDGTIL AQKLAEEVPM DVASYLYTGD SHQLKRANCS GRYELAGLPG KWPALASAHP121SLHRALDTLT HATNFLNVML QSNKSREQNL QDDLDWYQAL VWSLLEGEPS ISRAAITFST181DSLSAPAPQV FLQATREESR ILLQDLSSSA PHLANATLET EWFHGLRRKW RPHLHRRGPN241QGPRGLGHSW RRKDGLGGDK SHFKWSPPYL ECENGSYKPG WLVTLSSAIY GLQPNLVPEF301RGVMKVDINL QKVDIDQCSS DGWFSGTHKC HLNNSECMPI KGLGFVLGAY ECICKAGFYH361PGVLPVNNFR RRGPDQHISG STKDVSEEAY VCLPCREGCP FCADDSPCFV QEDKYLRLAI421ISFQALCMLL DFVSMLVVYH FRKAKSIRAS GLILLETILF GSLLLYFPVV ILYFEPSTFR481CILLRWARLL GFATVYGTVT LKLHRVLKVF LSRTAQRIPY MTGGRVMRML AVILLVVFWF541LIGWTSSVCQ NLEKQISLIG QGKTSDHLIF NMCLIDRWDY MTAVAEFLFL LWGVYLCYAV601RTVPSAFHEP RYMAVAVHNE LIISAIFHTI RFVLASRLQS DWMLMLYFAH THLTVTVTIG661LLLIPKFSHS SNNPRDDIAT EAYEDELDMG RSGSYLNSSI NSAWSEHSLD PEDIRDELKK721LYAQLEIYKR KKMITNNPHL QKKRCSKKGL GRSIMRRITE IPETVSRQCS KEDKEGADHG781TAKGTALIRK NPPESSGNTG KSKEETLKNR VFSLKKSHST YDHVRDQTEE SSSLPTESQE841EETTENSTLE SLSGKKLTQK LKEDSEAEST ESVPLVCKSA SAHNLSSEKK TGHPRTSMLQ901KSLSVIASAK EKTLGLAGKT QTAGVEERTK SQKPLPKDKE TNRNHSNSDN TETKDPAPQN961SNPAEEPRKP QKSGIMKQQR VNPTTANSDL NPGTTQMKDN FDIGEVCPWE VYDLTPGPVP1021SESKVQKHVS IVASEMEKNP TFSLKEKSHH KPKAAEVCQQ SNQKRIDKAE VCLWESQGQS1081ILEDEKLLIS KTPVLPERAK EENGGQPRAA NVCAGQSEEL PPKAVASKTE NENLNQIGHQ1141EKKTSSSEEN VRGSYNSSNN FQQPLTSRAE VCPWEFETPA QPNAGRSVAL PASSALSANK1201IAGPRKEEIW DSFKVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1215 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
