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GPR158 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR158 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPR158 antibodies →
$725.00each
Catalog Number: LS-G111060-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR158 (NM_020752), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1136 | G protein-coupled receptor 158 | GPR158 | RP11-59G22.1
Origin Species
Human
NCBI GenBank
NM_020752
SwissProt
Q5T848

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGAMAYPLLL CLLLAQLGLG AVGASRDPQG RPDSPRERTP KGKPHAQQPG RASASDSSAP
61WSRSTDGTIL AQKLAEEVPM DVASYLYTGD SHQLKRANCS GRYELAGLPG KWPALASAHP
121SLHRALDTLT HATNFLNVML QSNKSREQNL QDDLDWYQAL VWSLLEGEPS ISRAAITFST
181DSLSAPAPQV FLQATREESR ILLQDLSSSA PHLANATLET EWFHGLRRKW RPHLHRRGPN
241QGPRGLGHSW RRKDGLGGDK SHFKWSPPYL ECENGSYKPG WLVTLSSAIY GLQPNLVPEF
301RGVMKVDINL QKVDIDQCSS DGWFSGTHKC HLNNSECMPI KGLGFVLGAY ECICKAGFYH
361PGVLPVNNFR RRGPDQHISG STKDVSEEAY VCLPCREGCP FCADDSPCFV QEDKYLRLAI
421ISFQALCMLL DFVSMLVVYH FRKAKSIRAS GLILLETILF GSLLLYFPVV ILYFEPSTFR
481CILLRWARLL GFATVYGTVT LKLHRVLKVF LSRTAQRIPY MTGGRVMRML AVILLVVFWF
541LIGWTSSVCQ NLEKQISLIG QGKTSDHLIF NMCLIDRWDY MTAVAEFLFL LWGVYLCYAV
601RTVPSAFHEP RYMAVAVHNE LIISAIFHTI RFVLASRLQS DWMLMLYFAH THLTVTVTIG
661LLLIPKFSHS SNNPRDDIAT EAYEDELDMG RSGSYLNSSI NSAWSEHSLD PEDIRDELKK
721LYAQLEIYKR KKMITNNPHL QKKRCSKKGL GRSIMRRITE IPETVSRQCS KEDKEGADHG
781TAKGTALIRK NPPESSGNTG KSKEETLKNR VFSLKKSHST YDHVRDQTEE SSSLPTESQE
841EETTENSTLE SLSGKKLTQK LKEDSEAEST ESVPLVCKSA SAHNLSSEKK TGHPRTSMLQ
901KSLSVIASAK EKTLGLAGKT QTAGVEERTK SQKPLPKDKE TNRNHSNSDN TETKDPAPQN
961SNPAEEPRKP QKSGIMKQQR VNPTTANSDL NPGTTQMKDN FDIGEVCPWE VYDLTPGPVP
1021SESKVQKHVS IVASEMEKNP TFSLKEKSHH KPKAAEVCQQ SNQKRIDKAE VCLWESQGQS
1081ILEDEKLLIS KTPVLPERAK EENGGQPRAA NVCAGQSEEL PPKAVASKTE NENLNQIGHQ
1141EKKTSSSEEN VRGSYNSSNN FQQPLTSRAE VCPWEFETPA QPNAGRSVAL PASSALSANK
1201IAGPRKEEIW DSFKV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1215 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.