GPR149 / PGR10 Antibody (aa451-500)
Catalog Number: LS-C120691
2 sizes / from $341.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GPR149 / PGR10 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89064-20
Catalog Number: LS-G89064
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR149 (NM_001038705) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GPR149 | PGR10 | G protein-coupled receptor 149 | IEDA
- Origin Species
- Human
- NCBI GenBank
- NM_001038705
- SwissProt
- Q86SP6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLFLSNLST NDSSLWKENH NSTDLLNPPG TLNIYLFCLT CLMTFAALVG SIYSLISLLK61MQNRTVVSML VASWSVDDLM SVLSVTIFMF LQWPNEVPGY FQFLCTTSAL MYLCQGLSSN121LKATLLVSYN FYTMHRGVGS QTASRRSGQV LGVVLTVWAA SLLLSALPLC GWGAFVRTPW181GCLVDCSSSY VLFLSIVYAL AFGLLVGLSV PLTHRLLCSE EPPRLHSNYQ EISRGASIPG241TPPTAGRVVS LSPEDAPGPS LRRSGGCSPS SDTVFGPGAP AAAGAEACRR ENRGTLYGTR301SFTVSVAQKR FALILALTKV VLWLPMMMHM VVQNVVGFQS LPLETFSFLL TLLATTVTPV361FVLSKRWTHL PCGCIINCRQ NAYAVASDGK KIKRKGFEFN LSFQKSYGIY KIAHEDYYDD421DENSIFYHNL MNSECETTKD PQRDNRNIFN AIKVEISTTP SLDSSTQRGI NKCTNTDITE481AKQDSNNKKD AFSDKTGGDI NYEETTFSEG PERRLSHEES QKPDLSDWEW CRSKSERTPR541QRSGYALAIP LCAFQGTVSL HAPTGKTLSL STYEVSAEGQ KITPASKKIE VYRSKSVGHE601PNSEDSSSTF VDTSVKIHLE VLEICDNEEA LDTVSIISNI SQSSTQVRSP SLRYSRKENR661FVSCDLGETA SYSLFLPTSN PDGDINISIP DTVEAHRQNS KRQHQERDGY QEEIQLLNKA721YRKREEESKG STRTRPLEQKLISEEDLAANDILDYKDDDDKV
731 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
