Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR149 / PGR10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120562-100
Catalog Number: LS-G120562
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR149 (NM_001038705), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GPR149 | PGR10 | G protein-coupled receptor 149 | IEDA
- Origin Species
- Human
- NCBI GenBank
- NM_001038705
- SwissProt
- Q86SP6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLFLSNLST NDSSLWKENH NSTDLLNPPG TLNIYLFCLT CLMTFAALVG SIYSLISLLK61MQNRTVVSML VASWSVDDLM SVLSVTIFMF LQWPNEVPGY FQFLCTTSAL MYLCQGLSSN121LKATLLVSYN FYTMHRGVGS QTASRRSGQV LGVVLTVWAA SLLLSALPLC GWGAFVRTPW181GCLVDCSSSY VLFLSIVYAL AFGLLVGLSV PLTHRLLCSE EPPRLHSNYQ EISRGASIPG241TPPTAGRVVS LSPEDAPGPS LRRSGGCSPS SDTVFGPGAP AAAGAEACRR ENRGTLYGTR301SFTVSVAQKR FALILALTKV VLWLPMMMHM VVQNVVGFQS LPLETFSFLL TLLATTVTPV361FVLSKRWTHL PCGCIINCRQ NAYAVASDGK KIKRKGFEFN LSFQKSYGIY KIAHEDYYDD421DENSIFYHNL MNSECETTKD PQRDNRNIFN AIKVEISTTP SLDSSTQRGI NKCTNTDITE481AKQDSNNKKD AFSDKTGGDI NYEETTFSEG PERRLSHEES QKPDLSDWEW CRSKSERTPR541QRSGYALAIP LCAFQGTVSL HAPTGKTLSL STYEVSAEGQ KITPASKKIE VYRSKSVGHE601PNSEDSSSTF VDTSVKIHLE VLEICDNEEA LDTVSIISNI SQSSTQVRSP SLRYSRKENR661FVSCDLGETA SYSLFLPTSN PDGDINISIP DTVEAHRQNS KRQHQERDGY QEEIQLLNKA721YRKREEESKG STRTRPLEQKLISEEDLAANDILDYKDDDDKV
731 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.