GPR142 Antibody
Catalog Number: LS-C809453
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human GPR142 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G92403-20
Catalog Number: LS-G92403
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GPR142 (NM_181790) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 142 | GPR142 | PGR2
- Origin Species
- Human
- NCBI GenBank
- NM_181790
- SwissProt
- Q7Z601
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSIMMLPMEQ KIQWVPTSLQ DITAVLGTEA YTEEDKSMVS HAQKSQHSCL SHSRWLRSPQ61VTGGSWDLRI RPSKDSSSFR QAQCLRKDPG ANNHLESQGV RGTAGDADRE LRGPSEKATA121GQPRVTLLPT PHVSGLSQEF ESHWPEIAER SPCVAGVIPV IYYSVLLGLG LPVSLLTAVA181LARLATRTRR PSYYYLLALT ASDIIIQVVI VFAGFLLQGA VLARQVPQAV VRTANILEFA241ANHASVWIAI LLTVDRYTAL CHPLHHRAAS SPGRTRRAIA AVLSAALLTG IPFYWWLDMW301RDTDSPRTLD EVLKWAHCLT VYFIPCGVFL VTNSAIIHRL RRRGRSGLQP RVGKSTAILL361GITTLFTLLW APRVFVMLYH MYVAPVHRDW RVHLALDVAN MVAMLHTAAN FGLYCFVSKT421FRATVRQVIH DAYLPCTLAS QPEGMAAKPV MEPPGLPTGA EVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
462 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
