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GPR142 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR142 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104381-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR142 (NM_181790), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 142 | GPR142 | PGR2
Origin Species
Human
NCBI GenBank
NM_181790
SwissProt
Q7Z601

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSIMMLPMEQ KIQWVPTSLQ DITAVLGTEA YTEEDKSMVS HAQKSQHSCL SHSRWLRSPQ
61VTGGSWDLRI RPSKDSSSFR QAQCLRKDPG ANNHLESQGV RGTAGDADRE LRGPSEKATA
121GQPRVTLLPT PHVSGLSQEF ESHWPEIAER SPCVAGVIPV IYYSVLLGLG LPVSLLTAVA
181LARLATRTRR PSYYYLLALT ASDIIIQVVI VFAGFLLQGA VLARQVPQAV VRTANILEFA
241ANHASVWIAI LLTVDRYTAL CHPLHHRAAS SPGRTRRAIA AVLSAALLTG IPFYWWLDMW
301RDTDSPRTLD EVLKWAHCLT VYFIPCGVFL VTNSAIIHRL RRRGRSGLQP RVGKSTAILL
361GITTLFTLLW APRVFVMLYH MYVAPVHRDW RVHLALDVAN MVAMLHTAAN FGLYCFVSKT
421FRATVRQVIH DAYLPCTLAS QPEGMAAKPV MEPPGLPTGA EV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

462 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.