LSBio
Human GPC2 / Glypican 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GPC2 / Glypican 2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$153.00each
Catalog Number: LS-G94599-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Glypican 2(GPC2) (NM_152742) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Glypican 2 | Glypican 2 (cerebroglycan) | Glypican-2 | Cerebroglycan | GPC2
Origin Species
Human
SwissProt
Q8N158

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSALRPLLLL LLPLCPGPGP GPGSEAKVTR SCAETRQVLG ARGYSLNLIP PALISGEHLR
61VCPQEYTCCS SETEQRLIRE TEATFRGLVE DSGSFLVHTL AARHRKFDEF FLEMLSVAQH
121SLTQLFSHSY GRLYAQHALI FNGLFSRLRD FYGESGEGLD DTLADFWAQL LERVFPLLHP
181QYSFPPDYLL CLSRLASSTD GSLQPFGDSP RRLRLQITRT LVAARAFVQG LETGRNVVSE
241ALKVPVSEGC SQALMRLIGC PLCRGVPSLM PCQGFCLNVV RGCLSSRGLE PDWGNYLDGL
301LILADKLQGP FSFELTAESI GVKISEGLMY LQENSAKVSA QVFQECGPPD PVPARNRRAP
361PPREEAGRLW SMVTEEERPT TAAGTNLHRL VWELRERLAR MRGFWARLSL TVCGDSRMAA
421DASLEAAPCW TGAGRGRYLP PVVGGSPAEQ VNNPELKVDA SGPDVPTRRR RLQLRAATAR
481MKTAALGHDL DGQDADEDAS GSGGGQQYAD DWMAGAVAPP ARPPRPPYPP RRDGSGGKGG
541GGSARYNQGR SRSGGASIGF HTQTILILSL SALALLGPR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

579 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.