Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPC2 / Glypican 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106024-100
Catalog Number: LS-G106024
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glypican 2(GPC2) (NM_152742), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Glypican 2 | Glypican 2 (cerebroglycan) | Glypican-2 | Cerebroglycan | GPC2
- Origin Species
- Human
- SwissProt
- Q8N158
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSALRPLLLL LLPLCPGPGP GPGSEAKVTR SCAETRQVLG ARGYSLNLIP PALISGEHLR61VCPQEYTCCS SETEQRLIRE TEATFRGLVE DSGSFLVHTL AARHRKFDEF FLEMLSVAQH121SLTQLFSHSY GRLYAQHALI FNGLFSRLRD FYGESGEGLD DTLADFWAQL LERVFPLLHP181QYSFPPDYLL CLSRLASSTD GSLQPFGDSP RRLRLQITRT LVAARAFVQG LETGRNVVSE241ALKVPVSEGC SQALMRLIGC PLCRGVPSLM PCQGFCLNVV RGCLSSRGLE PDWGNYLDGL301LILADKLQGP FSFELTAESI GVKISEGLMY LQENSAKVSA QVFQECGPPD PVPARNRRAP361PPREEAGRLW SMVTEEERPT TAAGTNLHRL VWELRERLAR MRGFWARLSL TVCGDSRMAA421DASLEAAPCW TGAGRGRYLP PVVGGSPAEQ VNNPELKVDA SGPDVPTRRR RLQLRAATAR481MKTAALGHDL DGQDADEDAS GSGGGQQYAD DWMAGAVAPP ARPPRPPYPP RRDGSGGKGG541GGSARYNQGR SRSGGASIGF HTQTILILSL SALALLGPRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
579 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.