LSBio
Human GOLGA8B / Golgin-67 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GOLGA8B / Golgin-67 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GOLGA8B antibodies →
$153.00each
Catalog Number: LS-G91378-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GOLGA8B (NM_001023567) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GOLGA8B | GM88 autoantigen | Golgin subfamily A member 8B | Golgin-67 | KIAA0855 | Golgin A8 family | member B
Origin Species
Human
NCBI GenBank
AF204231
SwissProt
A8MQT2

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEETGQSKL AAAKKKFKEY WQRNRPGVPA AAKRNTKANG SSPETAASGG CHSSEASSSA
61SSSLHARQSP CQEQAAVLNS RSIKISRLND TIKSLKQQKK QVEHQLEEEK KANNEKQKAE
121RELEGQIQRL NTEKKKLNTD LYHMKHSLRY FEEESKDLAG RLQRSSQRIG ELEWSLCAVA
181ATQKKKPDGF SSRSKALLKR QLEQSIREQI LLKGHVTQLK ESLKEVQLER DQYAEQIKGE
241RAQWQQRMRK MSQEVCTLKE EKKHDTHRVE ELERSLSRLK NQMAEPLPPD APAVSSEVEL
301QDLRKELERV AGELQAQVEN NQCISLLNRG QKERLREQEE RLQEQQERLR EREKRLQQLA
361EPQSDLEELK HENKSALQLE QQVKELQEKL GQVMETLTSA EKEPEAAVPA SGTGGESSGL
421MDLLEEKADL REHVEKLELG FIQYRRERCH QKVHRLLTEP GDSAKDASPG GGHHQAGPGQ
481GGEEGEAAGA AGDGVAACGS YSEGHGKFLA AARNPAAEPS PGAPAPQELG AADKHGDLCE
541ASLTNSVEPA QGEAREGSSQ DNPTAQPVLQ LLGEMQDHQE HPGLGSNCCV PCFCWAWLPR
601RRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.