Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GOLGA8B / Golgin-67 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129064-100
Catalog Number: LS-G129064
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GOLGA8B (NM_001023567), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GOLGA8B | GM88 autoantigen | Golgin subfamily A member 8B | Golgin-67 | KIAA0855 | Golgin A8 family | member B
- Origin Species
- Human
- NCBI GenBank
- AF204231
- SwissProt
- A8MQT2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEETGQSKL AAAKKKFKEY WQRNRPGVPA AAKRNTKANG SSPETAASGG CHSSEASSSA61SSSLHARQSP CQEQAAVLNS RSIKISRLND TIKSLKQQKK QVEHQLEEEK KANNEKQKAE121RELEGQIQRL NTEKKKLNTD LYHMKHSLRY FEEESKDLAG RLQRSSQRIG ELEWSLCAVA181ATQKKKPDGF SSRSKALLKR QLEQSIREQI LLKGHVTQLK ESLKEVQLER DQYAEQIKGE241RAQWQQRMRK MSQEVCTLKE EKKHDTHRVE ELERSLSRLK NQMAEPLPPD APAVSSEVEL301QDLRKELERV AGELQAQVEN NQCISLLNRG QKERLREQEE RLQEQQERLR EREKRLQQLA361EPQSDLEELK HENKSALQLE QQVKELQEKL GQVMETLTSA EKEPEAAVPA SGTGGESSGL421MDLLEEKADL REHVEKLELG FIQYRRERCH QKVHRLLTEP GDSAKDASPG GGHHQAGPGQ481GGEEGEAAGA AGDGVAACGS YSEGHGKFLA AARNPAAEPS PGAPAPQELG AADKHGDLCE541ASLTNSVEPA QGEAREGSSQ DNPTAQPVLQ LLGEMQDHQE HPGLGSNCCV PCFCWAWLPR601RRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
603 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.