LSBio
Human GLIS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GLIS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GLIS1 antibodies →
$153.00each
Catalog Number: LS-G83537-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GLIS1 (NM_147193) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLIS1 | GLIS family zinc finger 1 | Zinc finger protein GLIS1 | GLI-similar 1
Origin Species
Human
NCBI GenBank
NM_147193
SwissProt
Q8NBF1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEARTSLSA HCRGPLATGL HPDLDLPGRS LATPAPSCYL LGSEPSSGLG LQPETHLPEG
61SLKRCCVLGL PPTSPASSSP CASSDVTSII RSSQTSLVTC VNGLRSPPLT GDLGGPSKRA
121RPGPASTDSH EGSLQLEACR KASFLKQEPA DEFSELFGPH QQGLPPPYPL SQLPPGPSLG
181GLGLGLAGRV VAGRQACRWV DCCAAYEQQE ELVRHIEKSH IDQRKGEDFT CFWAGCVRRY
241KPFNARYKLL IHMRVHSGEK PNKCMFEGCS KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ
301KAFSNSSDRA KHQRTHLDTK PYACQIPGCS KRYTDPSSLR KHVKAHSAKE QQVRKKLHAG
361PDTEADVLTE CLVLQQLHTS TQLAASDGKG GCGLGQELLP GVYPGSITPH NGLASGLLPP
421AHDVPSRHHP LDATTSSHHH LSPLPMAEST RDGLGPGLLS PIVSPLKGLG PPPLPPSSQS
481HSPGGQPFPT LPSKPSYPPF QSPPPPPLPS PQGYQGSFHS IQSCFPYGDC YRMAEPAAGG
541DGLVGETHGF NPLRPNGYHS LSTPLPATGY EALAEASCPT ALPQQPSEDV VSSGPEDCGF
601FPNGAFDHCL GHIPSIYTDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.