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GLIS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GLIS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106507-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLIS1 (NM_147193), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLIS1 | GLIS family zinc finger 1 | Zinc finger protein GLIS1 | GLI-similar 1
Origin Species
Human
NCBI GenBank
NM_147193
SwissProt
Q8NBF1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEARTSLSA HCRGPLATGL HPDLDLPGRS LATPAPSCYL LGSEPSSGLG LQPETHLPEG
61SLKRCCVLGL PPTSPASSSP CASSDVTSII RSSQTSLVTC VNGLRSPPLT GDLGGPSKRA
121RPGPASTDSH EGSLQLEACR KASFLKQEPA DEFSELFGPH QQGLPPPYPL SQLPPGPSLG
181GLGLGLAGRV VAGRQACRWV DCCAAYEQQE ELVRHIEKSH IDQRKGEDFT CFWAGCVRRY
241KPFNARYKLL IHMRVHSGEK PNKCMFEGCS KAFSRLENLK IHLRSHTGEK PYLCQHPGCQ
301KAFSNSSDRA KHQRTHLDTK PYACQIPGCS KRYTDPSSLR KHVKAHSAKE QQVRKKLHAG
361PDTEADVLTE CLVLQQLHTS TQLAASDGKG GCGLGQELLP GVYPGSITPH NGLASGLLPP
421AHDVPSRHHP LDATTSSHHH LSPLPMAEST RDGLGPGLLS PIVSPLKGLG PPPLPPSSQS
481HSPGGQPFPT LPSKPSYPPF QSPPPPPLPS PQGYQGSFHS IQSCFPYGDC YRMAEPAAGG
541DGLVGETHGF NPLRPNGYHS LSTPLPATGY EALAEASCPT ALPQQPSEDV VSSGPEDCGF
601FPNGAFDHCL GHIPSIYTDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.