LSBio
Human GCC1 / GCC88 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human GCC1 / GCC88 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all GCC1 antibodies →
$153.00each
Catalog Number: LS-G82106-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human GCC1 (NM_024523) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GCC1 | GCC1P | GCC88 | Golgi coiled-coil 1 | Golgi coiled-coil protein 1
Origin Species
Human
NCBI GenBank
NM_024523
SwissProt
Q96CN9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKFGMNFGG GPSKKDLLET IETQKKQLLQ YQARLKDVVR AYKSLLKEKE ALEASIKVLS
61VSHEADVGLA GVQLPGLTFP DSVDDRCSTH SEDSTGTATS LDTAASLTST KGEFGVEDDR
121PARGPPPPKS EEASWSESGV SSSSGDGPFA GGEVDKRLHQ LKTQLATLTS SLATVTQEKS
181RMEASYLADK KKMKQDLEDA SNKAEEERAR LEGELKGLQE QIAETKARLI TQQHDRAQEQ
241SDHALMLREL QKLLQEERTQ RQDLELRLEE TREALAGRAY AAEQMEGFEL QTKQLTREVE
301ELKSELQAIR DEKNQPDPRL QELQEEAARL KSHFQAQLQQ EMRKTALAED QLRQQSQVEE
361QRVAALENQI SEVSELLGTY EKAKQKDQLA IQKLKERILQ LDLENKTLAL AASSRSPLDS
421HGEESSLDVN VLKDKMEKLK RLLQVAARKS QVTLDVEKLC DLEIMPSSEA ADGEKATALY
481YQQELKQLKE EFERYKMRAQ VVLKSKNTKD GNLGKELEAA QEQLAELKEK YISLRLSCEE
541LEHQHQQEAD DWKQELARLQ QLHRQELERC QLDFRDRTLK LEEELHKQRD RALAVLTEKD
601LELEQLRSVA LASGLPGRRS PVGGGGPGDP ADTSSSDSLT QALQLAAANE PTFFLYAEQL
661ARKEVEITSL RKQKHRLEVE VHQLQDRLLE EGERHREEVA ALQSHIEKNI RDQSREGANL
721EYLKNIIYRF LTLPDSLGRQ QTLTAILTIL HFSPEEKQVI MRLPTSASWW PSGKR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

775 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.