Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GCC1 / GCC88 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109955-100
Catalog Number: LS-G109955
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GCC1 (NM_024523), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCC1 | GCC1P | GCC88 | Golgi coiled-coil 1 | Golgi coiled-coil protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_024523
- SwissProt
- Q96CN9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKFGMNFGG GPSKKDLLET IETQKKQLLQ YQARLKDVVR AYKSLLKEKE ALEASIKVLS61VSHEADVGLA GVQLPGLTFP DSVDDRCSTH SEDSTGTATS LDTAASLTST KGEFGVEDDR121PARGPPPPKS EEASWSESGV SSSSGDGPFA GGEVDKRLHQ LKTQLATLTS SLATVTQEKS181RMEASYLADK KKMKQDLEDA SNKAEEERAR LEGELKGLQE QIAETKARLI TQQHDRAQEQ241SDHALMLREL QKLLQEERTQ RQDLELRLEE TREALAGRAY AAEQMEGFEL QTKQLTREVE301ELKSELQAIR DEKNQPDPRL QELQEEAARL KSHFQAQLQQ EMRKTALAED QLRQQSQVEE361QRVAALENQI SEVSELLGTY EKAKQKDQLA IQKLKERILQ LDLENKTLAL AASSRSPLDS421HGEESSLDVN VLKDKMEKLK RLLQVAARKS QVTLDVEKLC DLEIMPSSEA ADGEKATALY481YQQELKQLKE EFERYKMRAQ VVLKSKNTKD GNLGKELEAA QEQLAELKEK YISLRLSCEE541LEHQHQQEAD DWKQELARLQ QLHRQELERC QLDFRDRTLK LEEELHKQRD RALAVLTEKD601LELEQLRSVA LASGLPGRRS PVGGGGPGDP ADTSSSDSLT QALQLAAANE PTFFLYAEQL661ARKEVEITSL RKQKHRLEVE VHQLQDRLLE EGERHREEVA ALQSHIEKNI RDQSREGANL721EYLKNIIYRF LTLPDSLGRQ QTLTAILTIL HFSPEEKQVI MRLPTSASWW PSGKRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
775 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.