FZD10 / Frizzled 10 Antibody
Catalog Number: LS-C406085
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FZD10 / Frizzled 10 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G88866-20
Catalog Number: LS-G88866
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Frizzled 10(FZD10) (NM_007197) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD350 antigen | CD350 | FZ-10 | FZD10 | Frizzled homolog 10 | HFz10 | Frizzled 10 | Frizzled family receptor 10 | Frizzled-10 | FzE7 | Fz10
- Origin Species
- Human
- NCBI GenBank
- NM_007197
- SwissProt
- Q9ULW2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQRPGPRLWL VLQVMGSCAA ISSMDMERPG DGKCQPIEIP MCKDIGYNMT RMPNLMGHEN61QREAVIQLHE FAPLVEYGCH GHLRFFLCSL YAPMCTEQVS TPIPACRVMC EQARLKCSPI121MEQFNFKWPD SLDCRKLPNK NDPNYLCMEA PNNGSDEPTR GSGLFPPLFR PQRPHSAQEH181PLKDGGPGRG GCDNPGKFHH VEKSASCAPL CTPGVDVYWS REDKRFAVVW LAIWAVLCFF241SSAFTVLTFL IDPARFRYPE RPIIFLSMCY CVYSVGYLIR LFAGAESIAC DRDSGQLYVI301QEGLESTGCT LVFLVLYYFG MASSLWWVVL TLTWFLAAGK KWGHEAIEAN SSYFHLAAWA361IPAVKTILIL VMRRVAGDEL TGVCYVGSMD VNALTGFVLI PLACYLVIGT SFILSGFVAL421FHIRRVMKTG GENTDKLEKL MVRIGLFSVL YTVPATCVIA CYFYERLNMD YWKILAAQHK481CKMNNQTKTL DCLMAASIPA VEIFMVKIFM LLVVGITSGM WIWTSKTLQS WQQVCSRRLK541KKSRRKPASV ITSGGIYKKA QHPQKTHHGK YEIPAQSPTC VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
