Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FZD10 / Frizzled 10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114795-100
Catalog Number: LS-G114795
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Frizzled 10(FZD10) (NM_007197), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD350 antigen | CD350 | FZ-10 | FZD10 | Frizzled homolog 10 | HFz10 | Frizzled 10 | Frizzled family receptor 10 | Frizzled-10 | FzE7 | Fz10
- Origin Species
- Human
- NCBI GenBank
- NM_007197
- SwissProt
- Q9ULW2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQRPGPRLWL VLQVMGSCAA ISSMDMERPG DGKCQPIEIP MCKDIGYNMT RMPNLMGHEN61QREAVIQLHE FAPLVEYGCH GHLRFFLCSL YAPMCTEQVS TPIPACRVMC EQARLKCSPI121MEQFNFKWPD SLDCRKLPNK NDPNYLCMEA PNNGSDEPTR GSGLFPPLFR PQRPHSAQEH181PLKDGGPGRG GCDNPGKFHH VEKSASCAPL CTPGVDVYWS REDKRFAVVW LAIWAVLCFF241SSAFTVLTFL IDPARFRYPE RPIIFLSMCY CVYSVGYLIR LFAGAESIAC DRDSGQLYVI301QEGLESTGCT LVFLVLYYFG MASSLWWVVL TLTWFLAAGK KWGHEAIEAN SSYFHLAAWA361IPAVKTILIL VMRRVAGDEL TGVCYVGSMD VNALTGFVLI PLACYLVIGT SFILSGFVAL421FHIRRVMKTG GENTDKLEKL MVRIGLFSVL YTVPATCVIA CYFYERLNMD YWKILAAQHK481CKMNNQTKTL DCLMAASIPA VEIFMVKIFM LLVVGITSGM WIWTSKTLQS WQQVCSRRLK541KKSRRKPASV ITSGGIYKKA QHPQKTHHGK YEIPAQSPTC VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.