LSBio
Human FSCB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FSCB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FSCB antibodies →
$153.00each
Catalog Number: LS-G92119-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FSCB (NM_032135) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf155 | DKFZP434F1017 | FSCB
Origin Species
Human
NCBI GenBank
NM_032135
SwissProt
Q5H9T9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGKSQQTDV IEKKKHMAIP KSSSPKATHR IGNTSGSKGS YSAKAYESIR VSSELQQTWT
61KRKHGQEMTS KSLQTDTIVE EKKEVKLVEE TVVPEEKSAD VREAAIELPE SVQDVEIPPN
121IPSVQLKMDR SQQTSRTGYW TMMNIPPVEK VDKEQQTYFS ESEIVVISRP DSSSTKSKED
181ALKHKSSGKI FASEQPEFQP ATNSNEEIGQ KNISRTSFTQ ETKKGPPVLL EDELREEVTV
241PVVQEGSAVK KVASAEIEPP STEKFPAKIQ PPLVEEATAK AEPRPAEETH VQVQPSTEET
301PDAEAATAVA ENSVKVQPPP AEEAPLVEFP AEIQPPSAEE SPSVELLAEI LPPSAEESLS
361EEPPAEILPP PAEKSPSVEP LGEIRSPSAQ KAPIEVQPLP AEGALEEASA KVEPPTVEET
421LADVQPLLPE EAPREEAREL QLSTAMETPA EEAPTEFQSP LPKETTAEEA SAEIQLLAAT
481EPPADETPAE ARSPLSEETS AEEAHAEVQS PLAEETTAEE ASAEIQLLAA IEAPADETPA
541EAQSPLSEET SAEEAPAEVQ SPSAKGVSIE EAPLELQPPS GEETTAEEAS AAIQLLAATE
601ASAEEAPAEV QPPPAEEAPA EVQPPPAEEA PAEVQPPPAE ETPAEVQPPP AEEAPAEVQP
661PPAEEAPAEV QPPPAEEAPA EVQSLPAEET PIEETLAAVH SPPADDVPAE EASVDKHSPP
721ADLLLTEEFP IGEASAEVSP PPSEQTPEDE ALVENVSTEF QSPQVAGIPA VKLGSVVLEG
781EAKFEEVSKI NSVLKDLSNT NDGQAPTLEI ESVFHIELKQ RPPEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

825 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.