LSBio
FSCB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FSCB Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109022-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FSCB (NM_032135), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf155 | DKFZP434F1017 | FSCB
Origin Species
Human
NCBI GenBank
NM_032135
SwissProt
Q5H9T9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGKSQQTDV IEKKKHMAIP KSSSPKATHR IGNTSGSKGS YSAKAYESIR VSSELQQTWT
61KRKHGQEMTS KSLQTDTIVE EKKEVKLVEE TVVPEEKSAD VREAAIELPE SVQDVEIPPN
121IPSVQLKMDR SQQTSRTGYW TMMNIPPVEK VDKEQQTYFS ESEIVVISRP DSSSTKSKED
181ALKHKSSGKI FASEQPEFQP ATNSNEEIGQ KNISRTSFTQ ETKKGPPVLL EDELREEVTV
241PVVQEGSAVK KVASAEIEPP STEKFPAKIQ PPLVEEATAK AEPRPAEETH VQVQPSTEET
301PDAEAATAVA ENSVKVQPPP AEEAPLVEFP AEIQPPSAEE SPSVELLAEI LPPSAEESLS
361EEPPAEILPP PAEKSPSVEP LGEIRSPSAQ KAPIEVQPLP AEGALEEASA KVEPPTVEET
421LADVQPLLPE EAPREEAREL QLSTAMETPA EEAPTEFQSP LPKETTAEEA SAEIQLLAAT
481EPPADETPAE ARSPLSEETS AEEAHAEVQS PLAEETTAEE ASAEIQLLAA IEAPADETPA
541EAQSPLSEET SAEEAPAEVQ SPSAKGVSIE EAPLELQPPS GEETTAEEAS AAIQLLAATE
601ASAEEAPAEV QPPPAEEAPA EVQPPPAEEA PAEVQPPPAE ETPAEVQPPP AEEAPAEVQP
661PPAEEAPAEV QPPPAEEAPA EVQSLPAEET PIEETLAAVH SPPADDVPAE EASVDKHSPP
721ADLLLTEEFP IGEASAEVSP PPSEQTPEDE ALVENVSTEF QSPQVAGIPA VKLGSVVLEG
781EAKFEEVSKI NSVLKDLSNT NDGQAPTLEI ESVFHIELKQ RPPEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

825 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.