LSBio
Human FOXN3 / CHES1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FOXN3 / CHES1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FOXN3 antibodies →
$153.00each
Catalog Number: LS-G75154-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human CHES1(FOXN3) (NM_005197) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf116 | Checkpoint suppressor 1 | CHES1 | Forkhead box N3 | FOXN3 | Forkhead box protein N3 | PRO1635
Origin Species
Human
NCBI GenBank
NM_005197
SwissProt
O00409

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL EEGAMEDEEL
61TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH SDMPYDARQN PNCKPPYSFS
121CLIFMAIEDS PTKRLPVKDI YNWILEHFPY FANAPTGWKN SVRHNLSLNK CFKKVDKERS
181QSIGKGSLWC IDPEYRQNLI QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF
241KRNGALLQVP PGVIQNGARV LSRGLFPGVR PLPITPIGVT AAMRNGITSC RMRTESEPSC
301GSPVVSGDPK EDHNYSSAKS SNARSTSPTS DSISSSSSSA DDHYEFATKG SQEGSEGSEG
361SFRSHESPSD TEEDDRKHSQ KEPKDSLGDS GYASQHKKRQ HFAKARKVPS DTLPLKKRRT
421EKPPESDDEE MKEAAGSLLH LAGIRSCLNN ITNRTAKGQK EQKETTKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

468 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.