Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FOXN3 / CHES1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119903-100
Catalog Number: LS-G119903
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHES1(FOXN3) (NM_001085471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf116 | Checkpoint suppressor 1 | CHES1 | Forkhead box N3 | FOXN3 | Forkhead box protein N3 | PRO1635
- Origin Species
- Human
- NCBI GenBank
- NM_005197
- SwissProt
- O00409
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL EEGAMEDEEL61TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH SDMPYDARQN PNCKPPYSFS121CLIFMAIEDS PTKRLPVKDI YNWILEHFPY FANAPTGWKN SVRHNLSLNK CFKKVDKERS181QSIGKGSLWC IDPEYRQNLI QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF241KRNGALLQDP DIDAASAMML LNTPPEIQAG FPPGVIQNGA RVLSRGLFPG VRPLPITPIG301VTAAMRNGIT SCRMRTESEP SCGSPVVSGD PKEDHNYSSA KSSNARSTSP TSDSISSSSS361SADDHYEFAT KGSQEGSEGS EGSFRSHESP SDTEEDDRKH SQKEPKDSLG DSGYASQHKK421RQHFAKARKV PSDTLPLKKR RTEKPPESDD EEMKEAAGSL LHLAGIRSCL NNITNRTAKG481QKEQKETTKNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
490 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.