LSBio
Human FNDC3A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FNDC3A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FNDC3A antibodies →
$220.00each
Catalog Number: LS-G84509-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FNDC3A (NM_014923) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA203I16.1 | BA203I16.5 | FNDC3A | HUGO | KIAA0970 | RP11-203I16.5 | FNDC3
Origin Species
Human
NCBI GenBank
AB023187
SwissProt
Q9Y2H6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
125.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGPAQVPMM SPNGSVPPIY VPPGYAPQVI EDNGVRRVVV VPQAPEFHPG SHTVLHRSPH
61PPLPGFIPVP TMMPPPPRHM YSPVTGAGDM TTQYMPQYQS SQVYGDVDAH STHGRSNFRD
121ERSSKTYERL QKKLKDRQGT QKDKMSSPPS SPQKCPSPIN EHNGLIKGQI AGGINTGSAK
181IKSGKGKGGT QVDTEIEEKD EETKAFEALL SNIVKPVASD IQARTVVLTW SPPSSLINGE
241TDESSVPELY GYEVLISSTG KDGKYKSVYV GEETNITLND LKPAMDYHAK VQAEYNSIKG
301TPSEAEIFTT LSCEPDIPNP PRIANRTKNS LTLQWKAPSD NGSKIQNFVL EWDEGKGNGE
361FCQCYMGSQK QFKITKLSPA MGCKFRLSAR NDYGTSGFSE EVLYYTSGCA PSMPASPVLT
421KAGITWLSLQ WSKPSGTPSD EGISYILEME EETSGYGFKP KYDGEDLAYT VKNLRRSTKY
481KFKVIAYNSE GKSNPSEVVE FTTCPDKPGI PVKPSVKGKI HSHSFKITWD PPKDNGGATI
541NKYVVEMAEG SNGNKWEMIY SGATREHLCD RLNPGCFYRL RVYCISDGGQ SAVSESLLVQ
601TPAVPPGPCL PPRLQGRPKA KEIQLRWGPP LVDGGSPISC YSVEMSPIEK DEPREVYQGS
661EVECTVSSLL PGKTYSFRLR AANKMGFGPF SEKCDITTAP GPPDQCKPPQ VTCRSATCAQ
721VNWEVPLSNG TDVTEYRLEW GGVEGSMQIC YCGPGLSYEI KGLSPATTYY CRVQALSVVG
781AGPFSEVVAC VTPPSVPGIV TCLQEISDDE IENPHYSPST CLAISWEKPC DHGSEILAYS
841IDFGDKQSLT VGKVTSYIIN NLQPDTTYRI RIQALNSLGA GPFSHMIKLK TKPLPPDPPR
901LECVAFSHQN LKLKWGEGTP KTLSTDSIQY HLQMEDKNGR FVSLYRGPCH TYKVQRLNES
961TSYKFCIQAC NEAGEGPLSQ EYIFTTPKSV PAALKAPKIE KVNDHICEIT WECLQPMKGD
1021PVIYSLQVML GKDSEFKQIY KGPDSSFRYS SLQLNCEYRF RVCAIRQCQD SLGHQDLVGP
1081YSTTVLFISQ RTEPPASTNR DTVESTRTRR ALSDEQCAAV ILVLFAFFSI LIAFIIQYFV
1141IK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1142 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.