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FNDC3A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FNDC3A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113596-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FNDC3A (NM_014923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA203I16.1 | BA203I16.5 | FNDC3A | HUGO | KIAA0970 | RP11-203I16.5 | FNDC3
Origin Species
Human
NCBI GenBank
AB023187
SwissProt
Q9Y2H6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
125.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGPAQVPMM SPNGSVPPIY VPPGYAPQVI EDNGVRRVVV VPQAPEFHPG SHTVLHRSPH
61PPLPGFIPVP TMMPPPPRHM YSPVTGAGDM TTQYMPQYQS SQVYGDVDAH STHGRSNFRD
121ERSSKTYERL QKKLKDRQGT QKDKMSSPPS SPQKCPSPIN EHNGLIKGQI AGGINTGSAK
181IKSGKGKGGT QVDTEIEEKD EETKAFEALL SNIVKPVASD IQARTVVLTW SPPSSLINGE
241TDESSVPELY GYEVLISSTG KDGKYKSVYV GEETNITLND LKPAMDYHAK VQAEYNSIKG
301TPSEAEIFTT LSCEPDIPNP PRIANRTKNS LTLQWKAPSD NGSKIQNFVL EWDEGKGNGE
361FCQCYMGSQK QFKITKLSPA MGCKFRLSAR NDYGTSGFSE EVLYYTSGCA PSMPASPVLT
421KAGITWLSLQ WSKPSGTPSD EGISYILEME EETSGYGFKP KYDGEDLAYT VKNLRRSTKY
481KFKVIAYNSE GKSNPSEVVE FTTCPDKPGI PVKPSVKGKI HSHSFKITWD PPKDNGGATI
541NKYVVEMAEG SNGNKWEMIY SGATREHLCD RLNPGCFYRL RVYCISDGGQ SAVSESLLVQ
601TPAVPPGPCL PPRLQGRPKA KEIQLRWGPP LVDGGSPISC YSVEMSPIEK DEPREVYQGS
661EVECTVSSLL PGKTYSFRLR AANKMGFGPF SEKCDITTAP GPPDQCKPPQ VTCRSATCAQ
721VNWEVPLSNG TDVTEYRLEW GGVEGSMQIC YCGPGLSYEI KGLSPATTYY CRVQALSVVG
781AGPFSEVVAC VTPPSVPGIV TCLQEISDDE IENPHYSPST CLAISWEKPC DHGSEILAYS
841IDFGDKQSLT VGKVTSYIIN NLQPDTTYRI RIQALNSLGA GPFSHMIKLK TKPLPPDPPR
901LECVAFSHQN LKLKWGEGTP KTLSTDSIQY HLQMEDKNGR FVSLYRGPCH TYKVQRLNES
961TSYKFCIQAC NEAGEGPLSQ EYIFTTPKSV PAALKAPKIE KVNDHICEIT WECLQPMKGD
1021PVIYSLQVML GKDSEFKQIY KGPDSSFRYS SLQLNCEYRF RVCAIRQCQD SLGHQDLVGP
1081YSTTVLFISQ RTEPPASTNR DTVESTRTRR ALSDEQCAAV ILVLFAFFSI LIAFIIQYFV
1141IK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1142 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.