FBXW7 / FBW7 Antibody
Catalog Number: LS-C401510
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FBXW7 / FBW7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G90923-20
Catalog Number: LS-G90923
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FBXW7(FBXW7) (NM_001013415) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGO | Archipelago homolog | Archipelago | CDC4 | F-box protein FBW7 | FBXW6 | F-box protein FBX30 | F-box protein SEL-10 | FBXW7 | HCdc4 | Homolog of C elegans sel-10 | FBW6 | FBX30 | FBW7 | HAgo | SEL-10 | SEL10
- Origin Species
- Human
- NCBI GenBank
- NM_018315
- SwissProt
- Q969H0
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSKPGKPTLN HGLVPVDLKS AKEPLPHQTV MKIFSISIIA QGLPFCRRRM KRKLDHGSEV61RSFSLGKKPC KVSEYTSTTG LVPCSATPTT FGDLRAANGQ GQQRRRITSV QPPTGLQEWL121KMFQSWSGPE KLLALDELID SCEPTQVKHM MQVIEPQFQR DFISLLPKEL ALYVLSFLEP181KDLLQAAQTC RYWRILAEDN LLWREKCKEE GIDEPLHIKR RKVIKPGFIH SPWKSAYIRQ241HRIDTNWRRG ELKSPKVLKG HDDHVITCLQ FCGNRIVSGS DDNTLKVWSA VTGKCLRTLV301GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS361RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ421GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT GHQSLTSGME LKDNILVSGN481ADSTVKIWDI KTGQCLQTLQ GPNKHQSAVT CLQFNKNFVI TSSDDGTVKL WDLKTGEFIR541NLVTLESGGS GGVVWRIRAS NTKLVCAVGS RNGTEETKLL VLDFDVDMKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
589 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
