Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXW7 / FBW7 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111835-100
Catalog Number: LS-G111835
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXW7(FBXW7) (NM_018315), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGO | Archipelago homolog | Archipelago | CDC4 | F-box protein FBW7 | FBXW6 | F-box protein FBX30 | F-box protein SEL-10 | FBXW7 | HCdc4 | Homolog of C elegans sel-10 | FBW6 | FBX30 | FBW7 | HAgo | SEL-10 | SEL10
- Origin Species
- Human
- NCBI GenBank
- NM_018315
- SwissProt
- Q969H0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCVPRSGLIL SCICLYCGVL LPVLLPNLPF LTCLSMSTLE SVTYLPEKGL YCQRLPSSRT61HGGTESLKGK NTENMGFYGT LKMIFYKMKR KLDHGSEVRS FSLGKKPCKV SEYTSTTGLV121PCSATPTTFG DLRAANGQGQ QRRRITSVQP PTGLQEWLKM FQSWSGPEKL LALDELIDSC181EPTQVKHMMQ VIEPQFQRDF ISLLPKELAL YVLSFLEPKD LLQAAQTCRY WRILAEDNLL241WREKCKEEGI DEPLHIKRRK VIKPGFIHSP WKSAYIRQHR IDTNWRRGEL KSPKVLKGHD301DHVITCLQFC GNRIVSGSDD NTLKVWSAVT GKCLRTLVGH TGGVWSSQMR DNIIISGSTD361RTLKVWNAET GECIHTLYGH TSTVRCMHLH EKRVVSGSRD ATLRVWDIET GQCLHVLMGH421VAAVRCVQYD GRRVVSGAYD FMVKVWDPET ETCLHTLQGH TNRVYSLQFD GIHVVSGSLD481TSIRVWDVET GNCIHTLTGH QSLTSGMELK DNILVSGNAD STVKIWDIKT GQCLQTLQGP541NKHQSAVTCL QFNKNFVITS SDDGTVKLWD LKTGEFIRNL VTLESGGSGG VVWRIRASNT601KLVCAVGSRN GTEETKLLVL DFDVDMKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
627 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.