LSBio
Human FBXO18 / FBH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FBXO18 / FBH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FBXO18 antibodies →
$220.00each
Catalog Number: LS-G82184-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FBXO18 (NM_032807) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box DNA helicase 1 | FBXO18 | F-box only protein 18 | F-box protein | helicase | 18 | FBH1 | Fbx18
Origin Species
Human
NCBI GenBank
NM_032807
SwissProt
Q8NFZ0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYEVTSGCH WTCQVPESCD NGLHCAGPLG HLHRRCQRTS AHLLVFTEHA EMRRFKRKHL
61TAIDCQHLAR SHLAVTQPFG QRWTNRDPNH GLYPKPRTKR GSRGQGSQRC IPEFFLAGKQ
121PCTNDMAKSN SVGQDSCQDS EGDMIFPAES SCALPQEGSA GPGSPGSAPP SRKRSWSSEE
181ESNQATGTSR WDGVSKKAPR HHLSVPCTRP REARQEAEDS TSRLSAESGE TDQDAGDVGP
241DPIPDSYYGL LGTLPCQEAL SHICSLPSEV LRHVFAFLPV EDLYWNLSLV CHLWREIISD
301PLFIPWKKLY HRYLMNEEQA VSKVDGILSN CGIEKESDLC VLNLIRYTAT TKCSPSVDPE
361RVLWSLRDHP LLPEAEACVR QHLPDLYAAA GGVNIWALVA AVVLLSSSVN DIQRLLFCLR
421RPSSTVTMPD VTETLYCIAV LLYAMREKGI NISNRIHYNI FYCLYLQENS CTQATKVKEE
481PSVWPGKKTI QLTHEQQLIL NHKMEPLQVV KIMAFAGTGK TSTLVKYAEK WSQSRFLYVT
541FNKSIAKQAE RVFPSNVICK TFHSMAYGHI GRKYQSKKKL NLFKLTPFMV NSVLAEGKGG
601FIRAKLVCKT LENFFASADE ELTIDHVPIW CKNSQGQRVM VEQSEKLNGV LEASRLWDNM
661RKLGECTEEA HQMTHDGYLK LWQLSKPSLA SFDAIFVDEA QDCTPAIMNI VLSQPCGKIF
721VGDPHQQIYT FRGAVNALFT VPHTHVFYLT QSFRFGVEIA YVGATILDVC KRVRKKTLVG
781GNHQSGIRGD AKGQVALLSR TNANVFDEAV RVTEGEFPSR IHLIGGIKSF GLDRIIDIWI
841LLQPEEERRK QNLVIKDKFI RRWVHKEGFS GFKRYVTAAE DKELEAKIAV VEKYNIRIPE
901LVQRIEKCHI EDLDFAEYIL GTVHKAKGLE FDTVHVLDDF VKVPCARHNL PQLPHFRVES
961FSEDEWNLLY VAVTRAKKRL IMTKSLENIL TLAGEYFLQA ELTSNVLKTG VVRCCVGQCN
1021NAIPVDTVLT MKKLPITYSN RKENKGGYLC HSCAEQRIGP LAFLTASPEQ VRAMERTVEN
1081IVLPRHEALL FLVF
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1094 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.