Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXO18 / FBH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108629-100
Catalog Number: LS-G108629
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO18 (NM_032807), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box DNA helicase 1 | FBXO18 | F-box only protein 18 | F-box protein | helicase | 18 | FBH1 | Fbx18
- Origin Species
- Human
- NCBI GenBank
- NM_032807
- SwissProt
- Q8NFZ0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 123.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYEVTSGCH WTCQVPESCD NGLHCAGPLG HLHRRCQRTS AHLLVFTEHA EMRRFKRKHL61TAIDCQHLAR SHLAVTQPFG QRWTNRDPNH GLYPKPRTKR GSRGQGSQRC IPEFFLAGKQ121PCTNDMAKSN SVGQDSCQDS EGDMIFPAES SCALPQEGSA GPGSPGSAPP SRKRSWSSEE181ESNQATGTSR WDGVSKKAPR HHLSVPCTRP REARQEAEDS TSRLSAESGE TDQDAGDVGP241DPIPDSYYGL LGTLPCQEAL SHICSLPSEV LRHVFAFLPV EDLYWNLSLV CHLWREIISD301PLFIPWKKLY HRYLMNEEQA VSKVDGILSN CGIEKESDLC VLNLIRYTAT TKCSPSVDPE361RVLWSLRDHP LLPEAEACVR QHLPDLYAAA GGVNIWALVA AVVLLSSSVN DIQRLLFCLR421RPSSTVTMPD VTETLYCIAV LLYAMREKGI NISNRIHYNI FYCLYLQENS CTQATKVKEE481PSVWPGKKTI QLTHEQQLIL NHKMEPLQVV KIMAFAGTGK TSTLVKYAEK WSQSRFLYVT541FNKSIAKQAE RVFPSNVICK TFHSMAYGHI GRKYQSKKKL NLFKLTPFMV NSVLAEGKGG601FIRAKLVCKT LENFFASADE ELTIDHVPIW CKNSQGQRVM VEQSEKLNGV LEASRLWDNM661RKLGECTEEA HQMTHDGYLK LWQLSKPSLA SFDAIFVDEA QDCTPAIMNI VLSQPCGKIF721VGDPHQQIYT FRGAVNALFT VPHTHVFYLT QSFRFGVEIA YVGATILDVC KRVRKKTLVG781GNHQSGIRGD AKGQVALLSR TNANVFDEAV RVTEGEFPSR IHLIGGIKSF GLDRIIDIWI841LLQPEEERRK QNLVIKDKFI RRWVHKEGFS GFKRYVTAAE DKELEAKIAV VEKYNIRIPE901LVQRIEKCHI EDLDFAEYIL GTVHKAKGLE FDTVHVLDDF VKVPCARHNL PQLPHFRVES961FSEDEWNLLY VAVTRAKKRL IMTKSLENIL TLAGEYFLQA ELTSNVLKTG VVRCCVGQCN1021NAIPVDTVLT MKKLPITYSN RKENKGGYLC HSCAEQRIGP LAFLTASPEQ VRAMERTVEN1081IVLPRHEALL FLVFSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1094 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.