LSBio
Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111)

Oligomerization of FasL (h) efficiently triggers Jurkat cell death.Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (rec.) (rec.) or FasL, Soluble (human) (rec.) + TNF ligands enhancer (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric) (rec.), Fc:FasL, Soluble (human) and FasL, Soluble (human) + TNF ligands enhancer) kill Jurkat cells at IC50 <0.2ng/ml.

Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)-Human ACRP30headless aa 18-111)

Expression SystemHuman Embryonic Kidney 293
Format:Lyophilized
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$444.00each
Catalog Number: LS-G3967-10

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Overview

Description
Human FasL (aa 139-281) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG(R)-tag.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
ALPS1B | Apoptosis antigen ligand | APTL | CD95L | CD95 ligand | CD178 antigen | CD95-L | Fas antigen ligand | Fas ligand | FASL | TNFSF6 | APT1LG1 | CD178 | FASLG
Origin Species
Hu
SwissProt
P48023

Protein

Expression System
HEK293
Fusion Tag
FLAG
Molecular Weight
~40kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1aa 139-281

10 aa

Format

Purity
>95%
Conjugate/Label
Unconj
Endotoxin Level
< 0.02 EU/µg
Format
Lyophilized
Formulation
Lyophilized from PBS
Purity Measurement Method
SDS-PAGE

Storage & Handling

Storage Conditions
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.