Human FAM179A Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G73367-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human FAM179A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95049-20
Catalog Number: LS-G95049
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human FAM179A (NM_199280) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM179A | Protein FAM179A | LOC165186
- Origin Species
- Human
- NCBI GenBank
- NM_199280
- SwissProt
- Q6ZUX3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTRDDVPEA KVLVPVAVYC GSIPRTSAGP RVLPPGSINS SLPHGEGSLQ PEPRALLNNE61EPSQLLRGLG QLGGLKLDTP SKGWQARNGH PRNLRALSLG DQPLVLLPSP ESEANSVARD121TIQIKDKLKK RRLSEGLAAS SRASLDPGGG PQGVPLHSTI PRATSQRLLR VPRPMPLIQS181IPTTPEASGV KEKGLDLPGS IPGPHELRPG AQEAQISWQY LHCNDEKMQK SLGAIVIPPI241PKARTVAATP SRVPGSLPSP LPPGQGVLTG LRAPRTRLAR GSGPREKTPA SLEPKPLASP301IRDRPAAAKK PALPFSQSAP TLTAFSFDCA REACPPLKEE DQKEIGTKIQ VTISKSAREK361MQLKQMKEME LLRRLEEPRT GQELTSQCLG SQRAFMKEGL LPLRGSGTLS VPTRLSGPCR421NDVSIILRKW ASRASLPSIP ISRQEPRFAR HASANSLPAV LTLGSPEWEE EEEMDLRACK481ELRPFSNPEL GLRDALQCLN SSDWQMKEKG LVSIQRLAAC HSEVLTGKLH DVCLVVTGEV541TNLRSKVSHL AISTLGDLFQ ALKKNMDQEA EEIARCLLQK MADTNEFIQR AAGQSLRAMV601ENVTLARSLV VLTSAGVYHR NPLIRKYAAE HLSAVLEQIG AEKLLSGTRD STDMLVHNLV661RLAQDSNQDT RFYGRKMVNI LMANTKFDAF LKQSLPSYDL QKVMAAIKQQ GIEDNDELPS721AKGRKVLRSL VVCENGLPIK EGLSCNGPRL VGLRSTLQGR GEMVEQLREL TRLLEAKDFR781SRMEGVGQLL ELCKAKTELV TAHLVQVFDA FTPRLQDSNK KVNQWALESF AKMIPLLRES841LHPMLLSIII TVADNLNSKN SGIYAAAVAV LDAMVESLDN LCLLPALAGR VRFLSGRAVL901DVTDRLAVLV ASVYPRKPQA VERHVLPILW HFLNTATRNG TLPGPSGNIR GVVCRLSRSL961QEHMGSRLLD FAASQPKHVL KTLQELLDSE SLGGSRKATD RGVAPDSKTT GSSYPFQLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1019 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
