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FAM179A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM179A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM179A antibodies →
$725.00each
Catalog Number: LS-G103373-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM179A (NM_199280), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM179A | Protein FAM179A | LOC165186
Origin Species
Human
NCBI GenBank
NM_199280
SwissProt
Q6ZUX3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTRDDVPEA KVLVPVAVYC GSIPRTSAGP RVLPPGSINS SLPHGEGSLQ PEPRALLNNE
61EPSQLLRGLG QLGGLKLDTP SKGWQARNGH PRNLRALSLG DQPLVLLPSP ESEANSVARD
121TIQIKDKLKK RRLSEGLAAS SRASLDPGGG PQGVPLHSTI PRATSQRLLR VPRPMPLIQS
181IPTTPEASGV KEKGLDLPGS IPGPHELRPG AQEAQISWQY LHCNDEKMQK SLGAIVIPPI
241PKARTVAATP SRVPGSLPSP LPPGQGVLTG LRAPRTRLAR GSGPREKTPA SLEPKPLASP
301IRDRPAAAKK PALPFSQSAP TLTAFSFDCA REACPPLKEE DQKEIGTKIQ VTISKSAREK
361MQLKQMKEME LLRRLEEPRT GQELTSQCLG SQRAFMKEGL LPLRGSGTLS VPTRLSGPCR
421NDVSIILRKW ASRASLPSIP ISRQEPRFAR HASANSLPAV LTLGSPEWEE EEEMDLRACK
481ELRPFSNPEL GLRDALQCLN SSDWQMKEKG LVSIQRLAAC HSEVLTGKLH DVCLVVTGEV
541TNLRSKVSHL AISTLGDLFQ ALKKNMDQEA EEIARCLLQK MADTNEFIQR AAGQSLRAMV
601ENVTLARSLV VLTSAGVYHR NPLIRKYAAE HLSAVLEQIG AEKLLSGTRD STDMLVHNLV
661RLAQDSNQDT RFYGRKMVNI LMANTKFDAF LKQSLPSYDL QKVMAAIKQQ GIEDNDELPS
721AKGRKVLRSL VVCENGLPIK EGLSCNGPRL VGLRSTLQGR GEMVEQLREL TRLLEAKDFR
781SRMEGVGQLL ELCKAKTELV TAHLVQVFDA FTPRLQDSNK KVNQWALESF AKMIPLLRES
841LHPMLLSIII TVADNLNSKN SGIYAAAVAV LDAMVESLDN LCLLPALAGR VRFLSGRAVL
901DVTDRLAVLV ASVYPRKPQA VERHVLPILW HFLNTATRNG TLPGPSGNIR GVVCRLSRSL
961QEHMGSRLLD FAASQPKHVL KTLQELLDSE SLGGSRKATD RGVAPDSKTT GSSYPFQLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1019 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.