LSBio
Human FAM160B2 / RAI16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human FAM160B2 / RAI16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all FAM160B2 antibodies →
$220.00each
Catalog Number: LS-G91670-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RAI16(FAM160B2) (NM_022749) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RAI16 | Protein FAM160B2 | Retinoic acid induced 16
Origin Species
Human
NCBI GenBank
NM_022749
SwissProt
Q86V87

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSRLGALLQ EAVGAREPSI DLLQAFVEHW KGITHYYIES TDESTPAKKT DIPWRLKQML
61DILVYEEQQQ AAAGEAGPCL EYLLQHKILE TLCTLGKAEY PPGMRQQVFQ FFSKVLAQVQ
121HPLLHYLSVH RPVQKLLRLG GTASGSVTEK EEVQFTTVLC SKIQQDPELL AYILEGKKIV
181GRKKACGEPT ALPKDTTSHG DKDCSHDGAP ARPQLDGESC GAQALNSHMP AETEELDGGT
241TESNLITSLL GLCQSKKSRV ALKAQENLLL LVSMASPAAA TYLVQSSACC PAIVRHLCQL
301YRSMPVFLDP ADIATLEGIS WRLPSAPSDE ASFPGKEALA AFLGWFDYCD HLITEAHTVV
361ADALAKAVAE NFFVETLQPQ LLHVSEQSIL TSTALLTAML RQLRSPALLR EAVAFLLGTD
421RQPEAPGDNP HTLYAHLIGH CDHLSDEISI TTLRLFEELL QKPHEGIIHS LVLRNLEGRP
481YVAWGSPEPE SYEDTLDLEE DPYFTDSFLD SGFQTPAKPR LAPATSYDGK TAVTEIVNSF
541LCLVPEEAKT SAFLEETGYD TYVHDAYGLF QECSSRVASW GWPLTPTPLD PHEPERPFFE
601GHFLRVLFDR MSRILDQPYS LNLQVTSVLS RLALFPHPHI HEYLLDPYIS LAPGCRSLFS
661VLVRVIGDLM QRIQRVPQFP GKLLLVRKQL TGQAPGEQLD HQTLLQGVVV LEEFCKELAA
721IAFVKFPPHD PRQNVSPAPE GQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

743 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.