Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM160B2 / RAI16 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110273-100
Catalog Number: LS-G110273
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAI16(FAM160B2) (NM_022749), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RAI16 | Protein FAM160B2 | Retinoic acid induced 16
- Origin Species
- Human
- NCBI GenBank
- NM_022749
- SwissProt
- Q86V87
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLSRLGALLQ EAVGAREPSI DLLQAFVEHW KGITHYYIES TDESTPAKKT DIPWRLKQML61DILVYEEQQQ AAAGEAGPCL EYLLQHKILE TLCTLGKAEY PPGMRQQVFQ FFSKVLAQVQ121HPLLHYLSVH RPVQKLLRLG GTASGSVTEK EEVQFTTVLC SKIQQDPELL AYILEGKKIV181GRKKACGEPT ALPKDTTSHG DKDCSHDGAP ARPQLDGESC GAQALNSHMP AETEELDGGT241TESNLITSLL GLCQSKKSRV ALKAQENLLL LVSMASPAAA TYLVQSSACC PAIVRHLCQL301YRSMPVFLDP ADIATLEGIS WRLPSAPSDE ASFPGKEALA AFLGWFDYCD HLITEAHTVV361ADALAKAVAE NFFVETLQPQ LLHVSEQSIL TSTALLTAML RQLRSPALLR EAVAFLLGTD421RQPEAPGDNP HTLYAHLIGH CDHLSDEISI TTLRLFEELL QKPHEGIIHS LVLRNLEGRP481YVAWGSPEPE SYEDTLDLEE DPYFTDSFLD SGFQTPAKPR LAPATSYDGK TAVTEIVNSF541LCLVPEEAKT SAFLEETGYD TYVHDAYGLF QECSSRVASW GWPLTPTPLD PHEPERPFFE601GHFLRVLFDR MSRILDQPYS LNLQVTSVLS RLALFPHPHI HEYLLDPYIS LAPGCRSLFS661VLVRVIGDLM QRIQRVPQFP GKLLLVRKQL TGQAPGEQLD HQTLLQGVVV LEEFCKELAA721IAFVKFPPHD PRQNVSPAPE GQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
743 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.