LSBio
Human EXOC8 / EXO84 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human EXOC8 / EXO84 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all EXOC8 antibodies →
$153.00each
Catalog Number: LS-G84969-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EXOC8 (NM_175876) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EXO84 | Exo84p | Exocyst complex 84 kDa subunit | Exocyst complex 84-kDa subunit | Exocyst complex component 8 | EXOC8 | SEC84
Origin Species
Human
NCBI GenBank
NM_175876
SwissProt
Q8IYI6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMAMSDSGA SRLRRQLESG GFEARLYVKQ LSQQSDGDRD LQEHRQRIQA LAEETAQNLK
61RNVYQNYRQF IETAREISYL ESEMYQLSHL LTEQKSSLES IPLTLLPAAA AAGAAAASGG
121EEGVGGAGGR DHLRGQAGFF STPGGASRDG SGPGEEGKQR TLTTLLEKVE GCRHLLETPG
181QYLVYNGDLV EYDADHMAQL QRVHGFLMND CLLVATWLPQ RRGMYRYNAL YSLDGLAVVN
241VKDNPPMKDM FKLLMFPESR IFQAENAKIK REWLEVLEDT KRALSEKRRR EQEEAAAPRG
301PPQVTSKATN PFEDDEEEEP AVPEVEEEKV DLSMEWIQEL PEDLDVCIAQ RDFEGAVDLL
361DKLNHYLEDK PSPPPVKELR AKVEERVRQL TEVLVFELSP DRSLRGGPKA TRRAVSQLIR
421LGQCTKACEL FLRNRAAAVH TAIRQLRIEG ATLLYIHKLC HVFFTSLLET AREFEIDFAG
481TDSGCYSAFV VWARSAMGMF VDAFSKQVFD SKESLSTAAE CVKVAKEHCQ QLGDIGLDLT
541FIIHALLVKD IQGALHSYKE IIIEATKHRN SEEMWRRMNL MTPEALGKLK EEMKSCGVSN
601FEQYTGDDCW VNLSYTVVAF TKQTMGFLEE ALKLYFPELH MVLLESLVEI ILVAVQHVDY
661SLRCEQDPEK KAFIRQNASF LYETVLPVVE KRFEEGVGKP AKQLQDLRNA SRLIRVNPES
721TTSVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

725 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.