Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EXOC8 / EXO84 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104936-100
Catalog Number: LS-G104936
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EXOC8 (NM_175876), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EXO84 | Exo84p | Exocyst complex 84 kDa subunit | Exocyst complex 84-kDa subunit | Exocyst complex component 8 | EXOC8 | SEC84
- Origin Species
- Human
- NCBI GenBank
- NM_175876
- SwissProt
- Q8IYI6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMAMSDSGA SRLRRQLESG GFEARLYVKQ LSQQSDGDRD LQEHRQRIQA LAEETAQNLK61RNVYQNYRQF IETAREISYL ESEMYQLSHL LTEQKSSLES IPLTLLPAAA AAGAAAASGG121EEGVGGAGGR DHLRGQAGFF STPGGASRDG SGPGEEGKQR TLTTLLEKVE GCRHLLETPG181QYLVYNGDLV EYDADHMAQL QRVHGFLMND CLLVATWLPQ RRGMYRYNAL YSLDGLAVVN241VKDNPPMKDM FKLLMFPESR IFQAENAKIK REWLEVLEDT KRALSEKRRR EQEEAAAPRG301PPQVTSKATN PFEDDEEEEP AVPEVEEEKV DLSMEWIQEL PEDLDVCIAQ RDFEGAVDLL361DKLNHYLEDK PSPPPVKELR AKVEERVRQL TEVLVFELSP DRSLRGGPKA TRRAVSQLIR421LGQCTKACEL FLRNRAAAVH TAIRQLRIEG ATLLYIHKLC HVFFTSLLET AREFEIDFAG481TDSGCYSAFV VWARSAMGMF VDAFSKQVFD SKESLSTAAE CVKVAKEHCQ QLGDIGLDLT541FIIHALLVKD IQGALHSYKE IIIEATKHRN SEEMWRRMNL MTPEALGKLK EEMKSCGVSN601FEQYTGDDCW VNLSYTVVAF TKQTMGFLEE ALKLYFPELH MVLLESLVEI ILVAVQHVDY661SLRCEQDPEK KAFIRQNASF LYETVLPVVE KRFEEGVGKP AKQLQDLRNA SRLIRVNPES721TTSVVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
725 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.