EWSR1 / EWS Antibody
Catalog Number: LS-C346054
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human EWSR1 / EWS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G78676-20
Catalog Number: LS-G78676
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EWSR1 (NM_001163286) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EWS | RNA-binding protein EWS | BK984G1.4 | EWS oncogene | EWSR1
- Origin Species
- Human
- NCBI GenBank
- NM_005243
- SwissProt
- Q01844
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTDYSTYS QAAAQQGYSA YTAQPTQGYA QTTQAYGQQS YGTYGQPTDV SYTQAQTTAT61YGQTAYATSY GQPPTGYTTP TAPQAYSQPV QGYGTGAYDT TTATVTTTQA SYAAQSAYGT121QPAYPAYGQQ PAATAPTSYS STQPTSYDQS SYSQQNTYGQ PSSYGQQSSY GQQSSYGQQP181PTSYPPQTGS YSQAPSQYSQ QSSSYGQQSS FRQDHPSSMG VYGQESGGFS GPGENRSMSG241PDNRGRGRGG FDRGGMSRGG RGGGRGGMGS AGERGGFNKP GGPMDEGPDL DLGPPVDPDE301DSDNSAIYVQ GLNDSVTLDD LADFFKQCGV VKMNKRTGQP MIHIYLDKET GKPKGDATVS361YEDPPTAKAA VEWFDGKDFQ GSKLKVSLAR KKPPMNSMRG GLPPREGRGM PPPLRGGPGG421PGGPGGPMGR MGGRGGDRGG FPPRGPRGSR GNPSGGGNVQ HRAGDWQCPN PGCGNQNFAW481RTECNQCKAP KPEGFLPPPF PPPGGDRGRG GPGGMRGGRG GLMDRGGPGG MFRGGRGGDR541GGFRGGRGMD RGGFGGGRRG GPGGPPGPLM EQMGGRRGGR GGPGKMDKGE HRQERRDRPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
600 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
