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Human ETNPPL / AGXT2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ETNPPL / AGXT2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ETNPPL antibodies →
$153.00each
Catalog Number: LS-G87148-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AGXT2L1(ETNPPL) (NM_001146590) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGXT2L1
Origin Species
Human
NCBI GenBank
NM_031279
SwissProt
Q8TBG4

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCELYSKRDT LGLRKKHIGP SCKVFFASDP IKIVRAQRQY MFDENGEQYL DCINNVAHGV
61VKAALKQMEL LNTNSRFLHD NIVEYAKRLS ATLPEKLSVC YFTNSGSEAN DLALRLARQF
121RGHQDVITLD HAYHGHLSSL IEISPYKFQK GKDVKKEFVH VAPTPDTYRG KYREDHADSA
181SAYADEVKKI IEDAHNSGRK IAAFIAESMQ SCGGQIIPPA GYFQKVAEYV HGAGGVFIAD
241EVQVGFGRVG KHFWSFQMYG EDFVPDIVTM GKPMGNGHPV ACVVTTKEIA EAFSSSGMEY
301FNTYGGNPVS CAVGLAVLDI IENEDLQGNA KRVGNYLTEL LKKQKAKHTL IGDIRGIGLF
361IGIDLVKDHL KRTPATAEAQ HIIYKMKEKR VLLSADGPHR NVLKIKPPMC FTEEDAKFMV
421DQLDRILTVL EEAMGTKTES VTSENTPCKT KMLKEAHIEL LRDSTTDSKE NPSRKRNGMC
481TDTHSLLSKR LKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.