Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ETNPPL / AGXT2L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128136-100
Catalog Number: LS-G128136
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGXT2L1(ETNPPL) (NM_001146627), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGXT2L1
- Origin Species
- Human
- NCBI GenBank
- NM_031279
- SwissProt
- Q8TBG4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGHCHPGVVK AALKQMELLN TNSRFLHDNI VEYAKRLSAT LPEKLSVCYF TNSGSEANDL61ALRLARQFRG HQDVITLDHA YHGHLSSLIE ISPYKFQKGK DVKKEFVHVA PTPDTYRGKY121REDHADSASA YADEVKKIIE DAHNSGRKIA AFIAESMQSC GGQIIPPAGY FQKVAEYVHG181AGGVFIADEV QVGFGRVGKH FWSFQMYGED FVPDIVTMGK PMGNGHPVAC VVTTKEIAEA241FSSSGMEYFN TYGGNPVSCA VGLAVLDIIE NEDLQGNAKR VGNYLTELLK KQKAKHTLIG301DIRGIGLFIG IDLVKDHLKR TPATAEAQHI IYKMKEKRVL LSADGPHRNV LKIKPPMCFT361EEDAKFMVDQ LDRILTVLEE AMGTKTESVT SENTPCKTKM LKEAHIELLR DSTTDSKENP421SRKRNGMCTD THSLLSKRLK TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
441 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.