LSBio
Human ESRP1 / RBM35A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ESRP1 / RBM35A Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ESRP1 antibodies →
$153.00each
Catalog Number: LS-G90609-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human RBM35A(ESRP1) (NM_017697) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RBM35A | RMB35A | RNA binding motif protein 35A | ESRP1 | RNA-binding motif protein 35A | RNA-binding protein 35A
Origin Species
Human
NCBI GenBank
NM_017697
SwissProt
Q6NXG1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTASPDYLVV LFGITAGATG AKLGSDEKEL ILLFWKVVDL ANKKVGQLHE VLVRPDQLEL
61TEDCKEETKI DVESLSSASQ LDQALRQFNQ SVSNELNIGV GTSFCLCTDG QLHVRQILHP
121EASKKNVLLP ECFYSFFDLR KEFKKCCPGS PDIDKLDVAT MTEYLNFEKS SSVSRYGASQ
181VEDMGNIILA MISEPYNHRF SDPERVNYKF ESGTCSKMEL IDDNTVVRAR GLPWQSSDQD
241IARFFKGLNI AKGGAALCLN AQGRRNGEAL VRFVSEEHRD LALQRHKHHM GTRYIEVYKA
301TGEDFLKIAG GTSNEVAQFL SKENQVIVRM RGLPFTATAE EVVAFFGQHC PITGGKEGIL
361FVTYPDGRPT GDAFVLFACE EYAQNALRKH KDLLGKRYIE LFRSTAAEVQ QVLNRFSSAP
421LIPLPTPPII PVLPQQFVPP TNVRDCIRLR GLPYAATIED ILDFLGEFAT DIRTHGVHMV
481LNHQGRPSGD AFIQMKSADR AFMAAQKCHK KNMKDRYVEV FQCSAEEMNF VLMGGTLNRN
541GLSPPPCKLP CLSPPSYTFP APAAVIPTEA AIYQPSVILN PRALQPSTAY YPAGTQLFMN
601YTAYYPSPPG SPNSLGYFPT AANLSGVPPQ PGTVVRMQGL AYNTGVKEIL NFFQGYQYAT
661EDGLIHTNDQ ARTLPKEWVC I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

681 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.