Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ESRP1 / RBM35A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120661-100
Catalog Number: LS-G120661
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM35A(ESRP1) (NM_001034915), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RBM35A | RMB35A | RNA binding motif protein 35A | ESRP1 | RNA-binding motif protein 35A | RNA-binding protein 35A
- Origin Species
- Human
- NCBI GenBank
- NM_017697
- SwissProt
- Q6NXG1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTASPDYLVV LFGITAGATG AKLGSDEKEL ILLFWKVVDL ANKKVGQLHE VLVRPDQLEL61TEDCKEETKI DVESLSSASQ LDQALRQFNQ SVSNELNIGV GTSFCLCTDG QLHVRQILHP121EASKKNVLLP ECFYSFFDLR KEFKKCCPGS PDIDKLDVAT MTEYLNFEKS SSVSRYGASQ181VEDMGNIILA MISEPYNHRF SDPERVNYKF ESGTCSKMEL IDDNTVVRAR GLPWQSSDQD241IARFFKGLNI AKGGAALCLN AQGRRNGEAL VRFVSEEHRD LALQRHKHHM GTRYIEVYKA301TGEDFLKIAG GTSNEVAQFL SKENQVIVRM RGLPFTATAE EVVAFFGQHC PITGGKEGIL361FVTYPDGRPT GDAFVLFACE EYAQNALRKH KDLLGKRYIE LFRSTAAEVQ QVLNRFSSAP421LIPLPTPPII PVLPQQFVPP TNVRDCIRLR GLPYAATIED ILDFLGEFAT DIRTHGVHMV481LNHQGRPSGD AFIQMKSADR AFMAAQKCHK KNMKDRYVEV FQCSAEEMNF VLMGGTLNRN541GLSPPPCLSP PSYTFPAPAA VIPTEAAIYQ PSVILNPRAL QPSTAYYPAG TQLFMNYTAY601YPSPPGSPNS LGYFPTAANL SGVPPQPGTV VRMQGLAYNT GVKEILNFSQ GYQYATEDGL661IHTNDQARTL PKEWVCITRTRPLEQKLISEEDLAANDILDYKDDDDKV
677 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.