ERG Antibody
Catalog Number: LS-C806138
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ERG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79284-20
Catalog Number: LS-G79284
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ERG (NM_001136154) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ERG-3 | Ets-related | TMPRSS2/ERG fusion | Transcriptional regulator ERG | ERG | p55 | Transforming protein ERG
- Origin Species
- Human
- NCBI GenBank
- NM_004449
- SwissProt
- P11308
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIQTVPDPAA HIKEALSVVS EDQSLFECAY GTPHLAKTEM TASSSSDYGQ TSKMSPRVPQ61QDWLSQPPAR VTIKMECNPS QVNGSRNSPD ECSVAKGGKM VGSPDTVGMN YGSYMEEKHM121PPPNMTTNER RVIVPADPTL WSTDHVRQWL EWAVKEYGLP DVNILLFQNI DGKELCKMTK181DDFQRLTPSY NADILLSHLH YLRETPLPHL TSDDVDKALQ NSPRLMHARN TGGAAFIFPN241TSVYPEATQR ITTRPDLPYE PPRRSAWTGH GHPTPQSKAA QPSPSTVPKT EDQRPQLDPY301QILGPTSSRL ANPGSGQIQL WQFLLELLSD SSNSSCITWE GTNGEFKMTD PDEVARRWGE361RKSKPNMNYD KLSRALRYYY DKNIMTKVHG KRYAYKFDFH GIAQALQPHP PESSLYKYPS421DLPYMGSYHA HPQKMNFVAP HPPALPVTSS SFFAAPNPYW NSPTGGIYPN TRLPTSHMPS481HLGTYYSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
486 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
