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ERG Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ERG Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102534-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ERG (NM_182918), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ERG-3 | Ets-related | TMPRSS2/ERG fusion | Transcriptional regulator ERG | ERG | p55 | Transforming protein ERG
Origin Species
Human
NCBI GenBank
NM_004449
SwissProt
P11308

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MASTIKEALS VVSEDQSLFE CAYGTPHLAK TEMTASSSSD YGQTSKMSPR VPQQDWLSQP
61PARVTIKMEC NPSQVNGSRN SPDECSVAKG GKMVGSPDTV GMNYGSYMEE KHMPPPNMTT
121NERRVIVPAD PTLWSTDHVR QWLEWAVKEY GLPDVNILLF QNIDGKELCK MTKDDFQRLT
181PSYNADILLS HLHYLRETPL PHLTSDDVDK ALQNSPRLMH ARNTGGAAFI FPNTSVYPEA
241TQRITTRPDL PYEPPRRSAW TGHGHPTPQS KAAQPSPSTV PKTEDQRPQL DPYQILGPTS
301SRLANPGSGQ IQLWQFLLEL LSDSSNSSCI TWEGTNGEFK MTDPDEVARR WGERKSKPNM
361NYDKLSRALR YYYDKNIMTK VHGKRYAYKF DFHGIAQALQ PHPPESSLYK YPSDLPYMGS
421YHAHPQKMNF VAPHPPALPV TSSSFFAAPN PYWNSPTGGI YPNTRLPTSH MPSHLGTYY
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.