EPS15 Antibody
Catalog Number: LS-C830048
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human EPS15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G75763-20
Catalog Number: LS-G75763
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EPS15 (NM_001159969) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AF-1P | AF1P | MLLT5 | Protein AF-1p | Protein Eps15 | EPS15
- Origin Species
- Human
- NCBI GenBank
- NM_001981
- SwissProt
- P42566
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYLKSDSGLG GWITIPAVAD VLKYSCIVCW SSREKNNVEQ DLKEKEDTIK QRTSEVQDLQ61DEVQRENTNL QKLQAQKQQV QELLDELDEQ KAQLEEQLKE VRKKCAEEAQ LISSLKAELT121SQESQISTYE EELAKAREEL SRLQQETAEL EESVESGKAQ LEPLQQHLQD SQQEISSMQM181KLMEMKDLEN HNSQLNWCSS PHSILVNGAT DYCSLSTSSS ETANLNEHVE GQSNLESEPI241HQESPARSSP ELLPSGVTDE NEVTTAVTEK VCSELDNNRH SKEEDPFNVD SSSLTGPVAD301TNLDFFQSDP FVGSDPFKDD PFGKIDPFGG DPFKGSDPFA SDCFFRQSTD PFATSSTDPF361SAANNSSITS VETLKHNDPF APGGTVVAAS DSATDPFASV FGNESFGGGF ADFSTLSKVN421NEDPFRSATS SSVSNVVITK NVFEETSVKS EDEPPALPPK IGTPTRPCPL PPGKRSINKL481DSPDPFKLND PFQPFPGNDS PKEKDPEIFC DPFTSATTTT NKEADPSNFA NFSAYPSEED541MIEWAKRESE REEEQRLARL NQQEQEDLEL AIALSKSEIS EATRTRPLEQKLISEEDLAANDILDYKDDDDKV
582 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
